摘要
目的:观察嗜酸乳杆菌(L.acidophilus)对感染人轮状病毒(humanrotavirus,HRV)乳鼠肠黏膜的保护作用.方法:将60只昆明种乳鼠随母鼠按窝随机均分为4组,给4日龄的乳鼠灌服HRV作为感染组,灌服HRV的前和后灌服L.acidophilus作为预防组和治疗组,正常对照组灌服同剂量的MEMEagle细胞培养液.在实验的第8天下午杀死每组9只乳鼠,取乳鼠盲肠内容物分离培养后,进行菌群分析;ELISA法检测肠黏膜分泌性免疫球蛋白(SIgA)、血清干扰素-r(IFN-γ)和肿瘤坏死因子-α(TNF-α);HE法染色乳鼠小肠黏膜切片,利用Image-ProPlus5.1图像软件分析各组乳鼠小肠黏膜厚度、绒毛高度和隐窝深度.结果:与正常对照组比较,HRV感染组乳鼠出现了肠道菌群失调、肠黏膜SIgA下降、血清IFN-γ和TNF-α上升、肠黏膜机械屏障受损,预防组和治疗组乳鼠肠道菌群失调、肠黏膜免疫力下降和肠黏膜机械屏障受损的情况均得到了改善.预防组与治疗组相比,前者对乳鼠肠黏膜受损的保护作用效果更好.结论:L.acidophilus对感染HRV乳鼠肠黏膜有一定的保护作用,感染前灌服L.acidophilus的保护效果好于感染后灌服.
AIM：To investigate the possible protective effect of Lactobacillus acidophilus（L.acidophilus） onintestinal mucosa of neonatal mice infected witha human rotavirus（HRV）.METHODS：Sixty 4-day-old Kunming micewere randomly and equally divided into control group,HRV-infected group,L.acidophiluspretreated group（treated before HRV infectionand L.acidophilus-treated group（treated afteHRV infection）.The symptoms in these micewere observed each day for 8 d.On day 8,nine mice of each group were sacrificed by cervical dislocation.Caecum samples were taken for bacterial isolation and culture to count bacterial colonies.Secretory IgA（sIgA） from the intestinal mucosa and serum interferon-γ（IFN-γ） and tumor necrosis factors-α（TNF-α） were detected by ELISA.The thickness of intestinal mucosa,height of villus and depth of crypt were measured using Image-Pro Plus 5.1 software.RESULTS：The mice in the normal control group did not suffer from diarrhea,whereas the HRVinfected group showed altered intestinal flora,decreased level of intestinal sIgA,increased serum IFN-γ and TNF-α levels,and damaged intestinal mucosal barrier.Compared to the HRV-infected group,mice of the L.acidophiluspretreated and-treated groups showed some amelioration in intestinal flora,intestinal sIgA,serum IFN-γ and TNF-α,and intestinal mucosal barrier.More obvious protective effect on intestinal mucosa was observed in the pretreatment group than in the treatment group.CONCLUSION：L.acidophilus possesses protective effect on intestinal mucosa of HRV-infected neonatal mice.Pretreatment with L.acidophilus has better protective effect than L.acidophilus treatment.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第28期2963-2969,共7页
World Chinese Journal of Digestology
关键词
轮状病毒
嗜酸乳杆菌
乳鼠
肠黏膜
Human rotavirus
L.acidophilus
Neonatal mice
Intestinal mucosa
