摘要
目的研究制备淋病奈瑟菌感染人癌胚抗原相关细胞黏附分子1（hCEACAMl）转基因小鼠模型的可行性。方法用hCEACAMl真核表达载体pCDPGICEAl，通过显微注射法制备转基因小鼠，PCR及序列分析法检测目的基因在小鼠基因组中的整合，Westernblot及FACS技术检测目的基因的表达，镜检及培养法检查淋病奈瑟菌对转基因小鼠的感染。结果产生的22只F0代小鼠中4只为转基因整合阳性，其中1只可表达hCEACAMl蛋白，且目的蛋白表达在细胞膜上。淋病奈瑟菌可在转基因小鼠中形成感染。结论hCEACAMl转基因小鼠可作为淋病奈瑟菌感染研究的转基因动物模型。
Objective To develop a transgenic mouse model for N. gonorrhoeae researches. Methods Human carcinoembryonic antigen-related cellular adhesion molecules 1 （ hCEACAM1 ） eukaryotic expression vector, pCDPGICAM1, was used to generate transgenic mice by microinjection. The funder mice were screened by PCR, sequence analysis, Western blot and fluorescence-activated cell sorting analysis, respectively. The transgenic mice expressing hCEACAMI were inoculated with N. gonorrhoeae intravaginally. Adhesion and infection of gonococci to mice were analyzed by bacteria culture and microscopy. Results Four （ lines 50, 53, 54, and 59） of the 22 F0 generation transgenic mice were found to carry the transgene. The hCEACAM1 protein was expressed on the cell membrane of various tissues in the line 53 transgenic mouse. Compared with normal mice, N. gonorrhoeae can successfully infect and cause inflammation in the transgenic mice. Conclusion The hCEACAM1 transgenic mouse can be used as an animal model for gonococcal infections.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2011年第10期907-911,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金项目（31172299,31100652）
江苏省自然科学基金项目（BK2009193,BK2009615,BK2010039,BY2009140）
