摘要
目的:研究宫颈鳞状上皮病变中细胞凋亡的情况及其意义。方法:选择2008年1月～2009年10月桂林医学院附属医院53例宫颈组织制成组织芯片,其中正常9例,CIN 32例,鳞状细胞癌12例;应用TUNEL法检测细胞凋亡情况。结果:凋亡阳性率依次为:正常组0%(0/9),CIN组15.62%(5/32),鳞状细胞癌66.67%(8/12);正常组、CIN组与癌症组凋亡阳性率比较差异有统计学意义(P<0.01)。结论:细胞凋亡现象随着宫颈鳞状上皮病变的发展逐渐明显,应用TUNEL法检测宫颈组织芯片,具有优质、高效、结果可靠的特点。
Objective：To study the condition and significance of apoptosis in cervical squamous epithelial lesions.Methods：53 cervical specimens were selected from the hospital from January 2008 to October 2009 to prepare tissue microarray,including 9 normal specimens,32 specimens of CIN and 12 specimens of cervical squamous cell carcinoma;TUNEL method was used to detect apoptosis.Results：The positive rates of apoptosis were 0%（0/9） in normal cervix group,15.62%（5/32） in CIN group,66.67%（8/12） in squamous cell carcinoma group;there was significant difference among the three groups（P0.01）.Conclusion：The phenomenon of apoptosis becomes clear with the aggravation of cervical squamous epithelial lesions,TUNEL technique has the advantages of high quality,high efficiency and reliability for tissue microarray detection.
出处
《中国妇幼保健》
CAS
北大核心
2011年第33期5235-5236,共2页
Maternal and Child Health Care of China
基金
2008年广西卫生厅自筹科研课题〔Z200827〕
桂林医学院基础医学院科研课题〔CKY200903〕
