摘要
目的:建立反相高效液相色谱同时检测水果罐头食品中苯甲酸(钠)、山梨酸(钾)、脱氢乙酸(钠)和甜味剂糖精钠的分析方法。方法:样品液调节pH7.0定容至适当体积后,超声提取10 min,最后经0.45μm针式滤头过滤,进10μl滤液上机检测。选用YWG-C18 4.6 mm×25 mm,10μm分析柱,甲醇:0.02 mol/L醋酸铵溶液(V∶V)=10∶90流动相,流速1.2 ml/min,波长230 nm下进行检测,以保留时间定性,峰面积定量。结果:一次进样分析仅需12 min。在1.0μg/ml～200.0μg/ml内线性正相关,R都在0.9995以上。苯甲酸、山梨酸、脱氢乙酸和糖精钠最低检测浓度依次分别为1.7 mg/kg、1.2 mg/kg、3.0 mg/kg、2.8 mg/kg,回收率为96%～110%,相对标准偏差(n=7)小于3%。结论:该法准确、快速、简单能够同时分析测定水果罐头中这四种食品添加剂。
Objective：To establish RP-HPLC method for simultaneous determination of benzoic acid（sodium）,sorbic acid（potassium）,dehydroepiandrosterone acetate（sodium） and the sweetener saccharin sodium in canned fruits.Methods： The sample buffer was regulated to pH7.0 with appropriate volume.After ultrasonic extraction for 10 min and filtration with 0.45 μm syringe-driven filter,10 μl filtrate was injected for detection.YWG-C18 4.6 mm×25 mm and 10 μm analytical column were selected for detection.The mobile phase was methanol and 0.02 mol/L ammonium acetate solution with the volume ratio of 10∶ 95.The flow rate was 1.2 ml/min and detection wavelength was 230 nm.The method was qualified by retention time and quantified by peak area.Results： Analysis of one injection only need 12 min.The linearity showed positive correlation in the range of 1.0 μg/ml^200.0 μg/ml with R above 0.9995.The lowest detection concentration of benzoic acid,sorbic acid,dehydroacetic acid and sodium saccharin were 1.7 mg/kg,1.2 mg/kg,3.0 mg/kg,2.8 mg/kg respectively.The recoveries were 96% to 110% and relative standard deviation（n=7） was less than 3%.Conclusion： This method is accurate,fast and simple,which can be used for determining the four food additives simultaneously in canned fruits.
出处
《中国卫生检验杂志》
CAS
2011年第11期2647-2649,共3页
Chinese Journal of Health Laboratory Technology
