摘要
[目的]测定并分析压砂甜瓜白粉病病原菌的18S rDNA序列。[方法]从宁夏中部干旱带压砂甜瓜主栽品种"玉金香"发病植株上分离白粉病病原菌,采用Chelex-100法从其分生孢子中提取基因组DNA,PCR扩增18S rDNA序列,测序后进行Blast分析比对,并构建系统发育树。[结果]18S rDNA序列分析表明压砂甜瓜白粉病病原菌属单囊壳属(Podosphaera)。[结论]为生物防治压砂甜瓜白粉病和抗白粉病育种研究提供了参考。
[Objective] The aim was to analyze the 18S rDNA sequence of melon powdery mildew from lands overlaid with sands.[Method] The pathogen of melon powdery mildew was isolated from infected plants of ＂Yujinxiang＂,a major melon variety cultivated in lands overlaid with sands of droughty midregion in Ningxia.Genome DNA was extracted from its conidia using Chelex-100 method.18S rDNA sequence was amplified by PCR,which was analyzed by Blast after sequencing,and the phylogenetic tree was constructed.[Result] 18S rDNA sequence analysis showed that the pathogen of melon powdery mildew belonged to Podosphaera.[Conclusion] The study provided reference for biocontrol and disease-resistance breeding against melon powdery mildew.
出处
《安徽农业科学》
CAS
北大核心
2011年第34期21072-21073,21076,共3页
Journal of Anhui Agricultural Sciences
基金
宁夏回族自治区自然科学基金项目(NZ0958)
