摘要
HND5具有内生性,并且对多种病原真菌有拮抗作用,是一株很有应用潜力的生防菌株。对该菌株核糖体内转录间隔区和β微管蛋白基因内转录间隔区进行了分析,其序列和紧密枝顶孢同源性最高。生物学特性研究表明,其生长最适培养基为PDA,最适温度为28℃,最适pH7,最适碳氮源分别为蔗糖和硝酸钠,菌株对重铬酸钾具有耐受性。
HND5 is an endophytic fungi and had antagonistic activity on varieties of pathogenic fungi,so it had application potential for biocontrol.Sequences analysis of ribosomal DNA and β-tubulin internal transcribed spacer region show HND5 had the highest homologous with Acremonium strictum.Biological characteristics research showed the optimum medium was PDA,the optimum temperature was 28℃,and the optimum pH was 7.The optimum carbon and nitrogen source were cane sugar and NaNO 3,respectively.HND5 had the tolerance for potassium dichromate.
出处
《中国农学通报》
CSCD
北大核心
2011年第27期272-276,共5页
Chinese Agricultural Science Bulletin
基金
中央级公益性科研院所基本科研业务费专项"内生拮抗真菌HND5防治香蕉根结线虫研究"(2009hzs1J005)
科技部科技人员服务企业项目"香蕉健康种苗标准化生产技术的应用及推广"(2009GJE20046)
中国热带农业科学院博士启动经费"未培养香蕉内生细菌类群多态性分析及其功能验证"(Hzs0601)
关键词
HND5
分子鉴定
生物学特性
HND5
molecular identification
biological characteristic