摘要
为了比较甲醇、甲醛和甲酸3种C1化合物对植物生理特性及基因表达的影响,更好地理解这3种C1化合物的作用机制,分别用5 mmol·L-1甲醇、甲醛、甲酸溶液均匀喷洒于温室条件下盆栽培养的拟南芥叶片上,以喷蒸馏水的植物为对照,每周喷1次,4周后分析施用这3种C1化合物对其生长及生理特性的影响.结果表明:甲醇促进拟南芥生长,而甲醛和甲酸抑制其生长,甲醛的抑制作用特别明显;甲醇处理显著降低了叶片花青素含量但提高了叶绿素a含量及叶绿素a/b的比值,甲醛处理显著降低了叶绿素a含量及叶绿素a/b的比值,甲酸处理显著提高了叶绿素a含量但大幅度降低了叶绿素b含量从而使叶绿素a/b的比值比对照高2倍;3种C1化合物处理都能增加叶片中可溶性总蛋白质含量,甲醇处理尤为明显,只有甲醛处理可显著提高叶片可溶性总糖、H2O2及羰基化蛋白质含量,产生氧化胁迫.对基因表达谱进行逆转录聚合酶链式反应(RT-PCR)的结果表明:所选的大多数光合作用相关基因的表达被甲醇诱导,而甲醛抑制它们的表达但诱导很多胁迫相关基因的表达,甲酸对大多数光合作用相关基因的表达没有显著影响;3种C1化合物对大多数C1代谢相关基因没有显著影响,但都抑制5,10-亚甲基-四氢叶酸还原酶基因的表达.
In order to compare the effects of methanol,formaldehyde and formic acid application on plant physiological characters and gene expressions and to better understand the functional mechanisms of the three C1 compounds,5 mmol·L-1 methanol,formaldehyde and formic acid were sprayed,respectively,on the leaves of Arabidopsis grown under greenhouse conditions once a week,using the plants sprayed with distilled water as the control.After four weeks,the effects of foliar spraying these C1 compounds on plant growth and physiological characters were analyzed.The results indicated that methanol stimulated the growth of Arabidopsis,whereas formaldehyde and formic acid inhibited its growth and the inhibition of formaldehyde was much remarkable.Application of methanol decreased anthocyanin content but increased chlorophyll（Chl） a content and a/b ratio significantly.On the contrary,formaldehyde treatment reduced Chl a content and a/b ratio markedly.Application of formic acid caused an obvious increase in Chl a content and a notable decrease in Chl b content,which led to 2-fold increase in Chl a/b ratio.Application of three C1 compounds all increased total leaf soluble protein content,and the effect of methanol spraying was especially remarkable.Only formaldehyde treatment elevated the levels of soluble sugars,H2O2 and carbonyl-proteins in leaves,suggesting occurrence of oxidative stress.Gene expression profiles by reverse transcription-polymerase chain reaction（RT-PCR） showed that methanol stimulation induced expressions of most selected photosynthesis-related genes,while formaldehyde repressed their expressions but up-regulated most of the selected stress-responsive genes.The expressions of most selected photosynthesis-related genes were not affected significantly by the application of formic acid.Interestingly,three C1 compounds had no remarkable impacts on the expressions of most selected genes involved in C1 metabolism,but all repressed the expression of 5,10-methylene-tetrahydrofolate（THF） reductase.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2011年第6期631-641,共11页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金资助项目(30970263)
云南省中青年学术与技术带头人培养经费资助项目(2004PY01-5)
关键词
甲醇
甲醛
甲酸
拟南芥
基因表达
methanol
formaldehyde
formic acid
Arabidopsis thaliana
gene expression
