摘要
通过对籼稻YTB成熟胚种子储存时间、预处理温度、消毒等预处理措施的系统试验,优化了愈伤组织诱导的种子预处理方法。精选当年产的新种子,于50℃去休眠48 h,只采用1.5%的次氯酸钠溶液消毒3次,每次15 min的消毒方法,即可达到提高愈伤组织诱导率的目的。简化后的消毒步骤,避免了酒精对种子内部结构的损伤,减少了氯化汞对环境的污染。
Based on pretreatment trail of indica rice YTB seeds, author systematically analyzed and optimized 3 pretreatment factors （storage time, pretreatment temperature and disinfection method） for mature embryos callus culture. The result showed that the callus induction rate was effectively improved by the following conditions ： carefully selecting seeds of the current year, pre-heating at 50 ℃ for 48 h and disinfecting with 1.5 % sodium hypochlorite solution for 3 - 15 min. The simplified disinfecting method could avoid the damage of alcohol to the seed internal structure and reduce the environmental pollution of mercuric chloride.
出处
《种子》
CSCD
北大核心
2011年第11期45-48,共4页
Seed
基金
中南民族大学自然科学基金(编号:YZY07007)
