摘要
为了比较CD3+CD56+NKT细胞与CD3+TCRVα24+iNKT细胞在外周血淋巴细胞中的相对比例及其表面分子表达的差别,本研究采集了健康人外周全血,用四色荧光抗体染色和流式细胞术检测CD3+CD56+NKT细胞和CD3+TCRVα24+iNKT细胞在淋巴细胞中的比例,及其亚群表型及活化分子CD69的表达情况。检测结果表明,在正常人外周血淋巴细胞中CD3+CD56+NKT细胞所占比例为3.90%±2.89%,以CD8亚群占多数(57.61%±17.35%);而CD3+TCRVα24+iNKT细胞所占比例仅为0.39%±0.19%,且以CD4亚群占多数(56.60%±19.66%)。两种NKT细胞的相对数量之间存在显著正相关(r=0.467,P<0.05),但两类细胞之间极少重叠。CD16和CD161在CD3+CD56+NKT细胞上的表达量显著高于在CD3+TCRVα24+iNKT细胞上的表达量(P均<0.01)。活化分子CD69在两种细胞上的表达量均较低(P>0.05)。本研究结果表明,正常人外周血CD3+CD56+NKT细胞与CD3+TCRVα24+iNKT细胞在相对数量、亚群及表型上存在显著差异,是两种截然不同的NKT细胞。
In order to compare the frequency and surface phenotype of CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋ invariant NKT（iNKT） cells in human peripheral blood lymphocytes,the peripheral whole blood samples from 23 healthy donors were obtained and stained with four-color fluorescence conjugated antibodies.The flow cytometry was performed to detect the frequency,surface phenotype of subsets and activation on CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋ iNKT cells.The results showed that the proportion of CD3＋CD56＋NKT cells in the peripheral blood lymphocytes was 3.90±2.89%,of which the proportion of CD8 positive subset was 7.61%±17.35%.Compared to CD3＋CD56＋NKT cells,the proportion of CD3＋ TCRVα24＋ iNKT cells was 0.39±0.19%,the major subset of iNKT cells was CD4 positive subset 56.60±19.66%.There was a positive correlation between the proportion of CD3＋CD56＋NKT cells and CD3＋ TCRVα24＋ iNKT cells（r=0.467,P0.05）,but little or no overlap between the two cell subsets.The expressions of CD16 and CD161 on CD3＋CD56＋NKT cells were 12.24±10.09% and 55.94±20.96%,respectively oth significantly higher than that on CD3＋ TCRVα24＋iNKT cells（3.2±2.38% and 33.95±14.14%,）（P0.01）.The expression of CD69 was both low on these two cell subsets.These results demonstrate the CD3＋CD56＋ NKT cells and CD3＋TCRVα24＋iNKT cells are two distinct NKT cell subsets owing to the significant differences of frequency and surface phenotype of subsets of the two cell subpopulations in human peripheral blood.
出处
《现代免疫学》
CAS
CSCD
北大核心
2011年第6期490-494,共5页
Current Immunology
基金
安徽省教育厅科研项目(KJ2011Z253)
蚌埠医学院科研项目(BY0917)
