摘要
根据侵染节瓜3种病毒——小西葫芦黄花叶病毒(ZYMV)、番木瓜环斑病毒(PRSV)和黄瓜花叶病毒(CMV)的外壳蛋白基因保守区序列设计特异引物,在建立各种病毒单项PCR技术的基础上,通过优化多重PCR反应条件,初步建立了能同步、快速、灵敏地检测这3种病毒的多重PCR检测体系,为节瓜病毒病原的检测提供了理论与技术支持。
According to the conserved nucleotide sequences of coat protein genes of Zucchini yellow mosaic virus（ZYMV）,Papaya ring spot virus（PRSV）and Cucumber mosaic virus（CMV）infected chieh-qua specific primers were designed. Based on the establishment of the optimal RT-PCR for the single detection of ZYMV,PRSV and CMV,a multiplex RT-PCR protocol for the detection of three viruses on chieh-qua was developed,which provided a simple,rapid and economic method for simultaneous detection of three chieh-qua viruses to support the pathogen detection of chieh-qua technically and theoretically.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第11期2215-2222,共8页
Acta Horticulturae Sinica
基金
广东省植物保护新技术重点实验室开放基金项目(植重2010-3)
关键词
节瓜
小西葫芦黄花叶病毒
番木瓜环斑病毒
黄瓜花叶病毒
多重PCR
chieh-qua
Benincasa hispida（Thunb.）Cogn. var. chieh-qua How.
Zucchini yellow mosaic virus
Papaya ring spot virus
Cucumber mosaic virus
multiplex RT-PCR
