摘要
本研究旨在建立转基因奶牛多重PCR快速检测方法,为转基因动物及产品出入境检测技术平台的建立提供技术支持,并为转基因动物及产品检测技术标准的制定提供参考。根据牛物种特异性基因(mtDNA)设计奶牛内源基因引物,根据外源基因人乳铁蛋白基因(human lactoferrin,hLF)和新霉素磷酸转移酶基因(NPTⅡ)设计特异性引物,优化反应条件,建立转基因奶牛多重PCR检测方法。本方法敏感、快速、特异,一个反应可以检测多个基因片段,可有效用于转基因奶牛外源基因的检测。
For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry﹠exit animal quarantine,detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study.The endogenous mtDNA gene specific for cow identification,exotic hLF gene,marker NPTⅡ gene were amplified according to different primers.The process of the multiplex PCR reaction was optimized.The system described herein represent simple,accurate,and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that can be reliably used for the identification of specific lines of transgenic cows.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第11期73-76,共4页
China Animal Husbandry & Veterinary Medicine
基金
转基因重大专项课题转基因产品检测新技术研究(2009ZX08012-001B)
