摘要
本文对鲤鱼卵黄中蛋白水解酶进行了研究,证明鲤鱼卵母细胞中存在蛋白水解酶,蛋白酶在pH3左右对卵黄蛋白产生水解作用,酶活性能被E-64和pepstatin抑制,说明蛋白酶分属于半胱氨酸蛋白酶类（又称巯基蛋白酶类）和天冬氨酸蛋白酶类（又称酸性蛋白酶类）。在pH4时,蛋白酶对牛血红蛋白具有较高的水解率。此外,对鲤鱼的卵黄蛋白进行了SDS-聚丙烯酰胺凝胶电泳。
The proteinases responsible for the degradation of yolk proteins in the eggs of Cyprinus carpio were studied. It was proofed that there were endogenous proteinases in the oocytes. The optimum pH of the proteinases to the yolk proteins was about 3. The activities of the proteinases could be inhibited by E-64 and pepstatin, which suggested the proteinases belonged to the cysteine proteinase family and aspartic proteinase family. The proteinases could hydrolyze the bovine hemoglobin at pH4. There was one major kind of protein in the yolk proteins, which was estimated as 110 kDa by SDS-PAGE. This protein was the major endosubstrate of the proteinases.
基金
山东大学青年科学基金
关键词
鲤鱼
卵黄蛋白水解酶
性质
Cyprinus carpio, Proteinases, Characterization
