摘要
Objective To determine whether extraadrenal tissues synthesize aldoster one in addition to vascular tissue and brain. Methods Ex vivo kidney perfusion was performed in normal Wistar rats, A CEI pretreated and adrenalectomized rats prior to the perfusion experiment. Afte r equilibration for 30 minutes, 120 ml of perfusate was collected and subjected to rever se phase HPLC and then aldosterone was measured by RIA. By RT PCR and Southern blot the expression of aldosterone synthase gene CYP11B2 mRNA was studied i n both kidney tissue and cultured renal tubular epithelial cell, lung and l iver tissues. In situ hybridization was used to identify the cell types of liver and lung expressing CYP11B2 mRNA.Results Production of aldosterone in the kidney perfusate was not chang ed in adrenalectomized rats although it was decreased in the group pretreated wi th ACEI perindopril. By RT PCR and Southern blot the expression of CYP11B2 mRNA was demonstrated in both kidney tissue and cultured renal tubular epithelial cell. We have also identified CYP11B2 mRNA expression in liver and lung of rats. In si tu hybridization showed that CYP11B2 mRNA was localized in the endoplasm of live r fat storing cell (Ito cells) and type Ⅱ alveolar cells of lung.Conclusions These studies prove that kidney, liver and lung are able to produce aldosterone.
Objective To determine whether extraadrenal tissues synthesize aldoster one in addition to vascular tissue and brain. Methods Ex vivo kidney perfusion was performed in normal Wistar rats, A CEI pretreated and adrenalectomized rats prior to the perfusion experiment. Afte r equilibration for 30 minutes, 120 ml of perfusate was collected and subjected to rever se phase HPLC and then aldosterone was measured by RIA. By RT PCR and Southern blot the expression of aldosterone synthase gene CYP11B2 mRNA was studied i n both kidney tissue and cultured renal tubular epithelial cell, lung and l iver tissues. In situ hybridization was used to identify the cell types of liver and lung expressing CYP11B2 mRNA.Results Production of aldosterone in the kidney perfusate was not chang ed in adrenalectomized rats although it was decreased in the group pretreated wi th ACEI perindopril. By RT PCR and Southern blot the expression of CYP11B2 mRNA was demonstrated in both kidney tissue and cultured renal tubular epithelial cell. We have also identified CYP11B2 mRNA expression in liver and lung of rats. In si tu hybridization showed that CYP11B2 mRNA was localized in the endoplasm of live r fat storing cell (Ito cells) and type Ⅱ alveolar cells of lung.Conclusions These studies prove that kidney, liver and lung are able to produce aldosterone.
