摘要
系统地研究了邻联甲苯胺法测定刺芹侧耳漆酶活力的影响因素。结果表明:波长620 nm、反应温度30℃、pH 4.2、邻联甲苯胺浓度5 mmol/L、反应时间3 min时,刺芹侧耳漆酶活力测定的灵敏度最高,酶活力最大,为3 033 U/(min.mL),是筛选前测定酶活力的1.98倍。
Influential factors of Laccase activity from trometry were investigated systematically. The results length was at 620 nm, temperature was at 30 °C, 5 mmol/L and reaction time was for 3 minutes when activity were the highest and laccase activity was up Pleurotus eryngii determined by ortho-tolidine specshowed that the optimum conditions were that wavepH was at 4.2, ortho-tolidine concentration was at both sensitivity rate of determination and the laccase to 3 033 U/（min mL）. It was 1.98 times of before.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2011年第6期620-623,共4页
Journal of Jilin Agricultural University
基金
吉林农业大学科研启动基金项目(201030)
关键词
漆酶
酶活力
邻联甲苯胺法
剌芹侧耳
laccase
enzyme activity
ortho-tolidine spectrometry
Pleurotus eryngii
