摘要
本研究对基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)的原理和应用做了简要介绍,并概括了常用基质的应用特点和适用范围。通过基质的选择及测试条件的优化,分别对3种席夫碱镍配合物进行质谱分析,实验结果表明,[M+H]+峰的分子质量以及同位素丰度都与理论计算值十分吻合。采用此法不仅能准确地测定席夫碱镍配合物的分子质量,为席夫碱镍配合物结构的鉴定奠定基础,也是金属有机化合物分子质量测定的有效方法,是快速分析金属有机化合物的工具之一。为了进一步说明质谱在金属配合物测定中的可靠性,辅以傅里叶变换离子回旋共振质谱仪(FT-ICR-MS)进行确证。
The principle and applications of matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS） were briefly introduced. The characteris- tics and scope of the application of matrixes were summarized. Three Schiff base nickel complexes were respectively analyzed by MALDI-TOF-MS. The results showed that the molecular weight [M＋H-]＋ peak and the isotopic abundance were very consistent with the theoretical value. By this method not only the molecular weight of Schiff base complexes was accurately determined, providing important assistance for the structural identification, but also it would be an effective and rapid tool for identification of metal organic complexes, and supplemented by Fourier transform ion cyclotron resonance mass spectrometer （FT- ICR-MS） for confirmation.
出处
《质谱学报》
EI
CAS
CSCD
2011年第5期288-292,300,共6页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金(2077309820973136)资助
