摘要
以菊花黄绿叶突变体金陵国紫为试验材料,对突变体黄绿叶中绿叶与黄叶组织的荧光参数、光能分配、非光化学猝灭系数(NPQ)及其三成分[高能态猝灭(qE)、状态转换猝灭(qT)和光抑制猝灭(qI)]和荧光诱导动力学曲线等进行了分析。结果表明:菊花突变体黄叶组织与绿叶组织相比,其初始荧光(Fo)较高,而最大光化学效率(Fv/Fm)、光下光系统II(PSⅡ)的最大光化学效率(Fv'/Fm')、PSⅡ实际光化学效率(ΦPSⅡ)、光化学淬灭系数(qP)等参数较低;在吸收光能分配上黄叶组织用于反应中心的过剩激发能(EX)与光化学反应的光能(P)远小于绿叶组织,而用于热耗散的光能(D)则远大于绿叶组织。黄叶组织中非光化学系数NPQ较高,与光抑制有关的NPQ成分qI增加较多。黄叶组织放氧复合体(OEC)可能受到伤害,光系统间初级醌电子受体(QA)与次级醌电子受体(QB)之间的电子传递受阻,单位面积有活性的反应中心数量(RC/CS)显著减少,单位面积捕获的光能(TRo/CS)与单位面积传递的光能(ETo/CS)显著低于绿叶组织,但单位面积的热耗散(DIo/CS)则大大高于绿叶组织,以吸收光能为基础的性能指数(PIABS)与绿叶组织相比也明显下降。说明黄叶组织的光合机构受到破坏,光化学能力下降;与绿叶组织相比,黄叶组织对光抑制比较敏感。黄叶组织通过提高热耗散能力,特别是依赖与光抑制相关的热耗散来减少过剩光能对黄叶组织光合机构的破坏。
A yellow-green leaf mutant of chrysanthemum,Jinglingguozi,was used to study the chlorophyll fluorescence parameters,absorbed light distribution,three components of non photochemical quenching coefficient（NPQ） as well as the parameters of fluorescence induction dynamics curve.Compared to the green-leaf tissue of the mutant,the yellow-leaf tissue had higher initial fluorescence（Fo）,and lower maximal photochemical efficiency（Fv / Fm）,photochemical efficiency of photosystem II（PSⅡ） in the light（Fv′/Fm′）,actual PSⅡ efficiency（ΦPSⅡ）,and photochemical quenching coefficients（qP）.The fraction of absorbed light allocated to excitation energy（EX） and photochemical reaction（P） in the yellow leaf tissue were far less than those in the green-leaf tissue,however,the fraction of absorbed light allocated to energy dissipation（D） was much more.NPQ related to heat excitation and the photoinhibitory quench（qI） of NPQ in the yellow-leaf tissue increased greatly.Oxygen-evolving complex（OEC） might be destroyed in the yellow-leaf tissue.PSⅡ electron transport between QA and QB was apparently blocked in the yellow-leaf tissue.Active reaction centers per cross-section（RC/CS） decreased obviously in the yellow-leaf tissue.Excited energy captured per cross-section（TRo/CS） and excited energy transferred per cross-section（ETo/CS） in the yellow-leaf tissue were greatly lower than those in the green leaf tissue,however,heat excitation per cross-section（DIo/CS） was greatly higher than in the green leaf tissue.Compared with the green-leaf tissue,the performance index on absorption basis（PIABS） reduced markedly in the yellow-leaf tissue.The comparison between the green-leaf tissue and the yellow-leaf tissue indicated the structure of photosystem might be destroyed in the yellow-leaf tissue.The photochemical efficiency of the yellow-leaf tissue reduced,and the yellow-leaf tissue was more sensitive to photoinhibition.The yellow-leaf tissue increased the heat dissipation,especially the heat excitation related to the photoinhibitory quench（qI）,to prevent the damage from overmuch excited energy to the photosynthetic apparatus.
出处
《江苏农业学报》
CSCD
北大核心
2011年第5期1093-1099,共7页
Jiangsu Journal of Agricultural Sciences
基金
农业部公益性行业(农业)科研专项(200903020)
科技部科技人员服务企业行动项目(2009GJC10039)
科技部农业科技成果转化资金项目(2010GB23600663)
关键词
菊花
突变体
叶绿素荧光
非光化学猝灭系数(NPQ)
chrysanthemum
mutant
chlorophyll fluorescence
non photochemical quenching coefficient（NPQ）
