摘要
为了筛选和评价稻曲病拮抗细菌,从云南、江苏两地采集的10份土样中分离获得1 815个细菌分离物,初筛采用平板对峙培养法测定获得的分离细菌对水稻纹枯病菌的拮抗性能,结果表明有83个菌株具有一定的拮抗活性。进一步测定了拮抗细菌对稻曲病菌、稻瘟病菌、水稻纹枯病菌、水稻细菌性条斑病菌和水稻白叶枯病菌的拮抗性能,并筛选获得了2株生长快、对稻曲病菌生长抑制作用强(抑制率分别达到97.2%和85.9%)及对其他水稻病原菌均表现出较强的抑菌活性的拮抗细菌SF-62和SF-3-38。田间试验结果表明,这2株拮抗细菌的发酵液对稻曲病病粒的防治效果分别为47.88%和43.12%。经16S rDNA方法鉴定,这2株拮抗细菌初步确定为枯草芽孢杆菌(Bacillus subtilis)。
To screen and evaluate of antagonistic bacteria against rice false smut,1 815 bacterial strains from 10 soil samples in Yunnan and Jangsu provinces were isolated,and the antagonism against Rhizoctonia solani was detected through diualculture on PDA plates.Among the tested strains,83 strains showed visible ability in inhibiting the growth of Rhizoctonia solani,of which two bacterium strains,SF-62 and SF-3-38,were found to have very high ability in inhibiting Ustilaginoidea virens,with the inhibition rates being 97.2% and 85.9%.They were also strongly antagonistic against,Pyricularia grisea,Xanthomonas oryzae pv.oryzicola and Xanthomonas oryzae pv.oryzae.In the field plots,the control efficacy of liquid fermentation of SF-62 and SF-3-38 on rice diseased kernel infected by Ustilaginoidea virens were 47.88% and 43.12%,respectively.Based on their 16S rDNA sequence analysis,SF-62 and SF-3-38 were both Bacillus subtilis.
出处
《江苏农业学报》
CSCD
北大核心
2011年第5期983-989,共7页
Jiangsu Journal of Agricultural Sciences
基金
公益性行业(农业)科研专项"稻曲病控制技术的研究"(200903039-2)
江苏省科技支撑计划项目(BE2011356)
关键词
稻曲菌
拮抗细菌
筛选
防效
Ustilaginoidea virens
antagonistic bacterium
screening
control efficacy
