摘要
采用UPLC-Q-TOF/MS研究四逆散提取物在大鼠体内的代谢产物,利用碰撞能量梯度(MSE)和质量亏损过滤(MDF)技术进行分析,鉴定大鼠灌胃四逆散提取物后血浆、尿液、粪便、胆汁中的代谢产物。四逆散提取物中柚皮苷、柚皮素、橙皮苷、新橙皮苷、甘草苷、甘草素、甘草酸、甘草次酸、柴胡皂苷a、柴胡皂苷d在大鼠不同代谢途径中推测出原形、羟基化、葡糖醛酸化、硫酸化、葡糖醛酸化与硫酸化结合、羟化葡糖醛酸化等共41个代谢产物。
Sinisan is a widely used traditional Chinese medicine（TCM） in treating various diseases;however,the in vivo metabolic profile of its multiple components remains unknown.In this paper,ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF/MS） was applied to identify the metabolites of Sinisan extract in rat plasma,urine,feces and bile after intragastric administration.Using MSE and mass defect filter techniques,41 metabolites of 10 parent compounds（naringin,naringenin,hesperidin,neohesperidin,liquiritin,liquiritigenin,glycyrrhizic acid,glycyrrhetinic acid,saikosaponin a and saikosaponin d） were detected and tentatively identified.It was shown by our results that these compounds was metabolized to the forms of hydroxylation,glucuronidation,sulfation,glucuronidation with sulfation and glucuronidation with hydroxylation in vivo.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第11期1374-1379,共6页
Acta Pharmaceutica Sinica
基金
江苏省方剂高技术研究重点实验室建设资助项目(BM2010576)
江苏省理血方剂创新药物工程中心建设资助项目
