摘要
探讨高翅果菊根茎中主要成分lactuside B对脑缺血再灌注损伤大鼠大脑皮质bcl-2、bax mRNA及其蛋白表达的影响。建立大鼠大脑中动脉缺血再灌注损伤模型,各组给予相应的实验药物,分别在缺血再灌注24和72 h处死所需动物,采用TTC染色法检测脑梗死体积,逆转录多聚酶链反应和免疫组化二步法分别检测bcl-2、bax mRNA及其蛋白在大鼠大脑皮质的表达情况。结果表明,lactuside B各剂量组均可缩小大鼠脑皮质的梗死体积,提高bcl-2 mRNA的表达、降低bax mRNA的表达,且12.5和25 mg.kg-1剂量组bcl-2、bax mRNA的比值较高,与模型组比较有显著性差异(P<0.05或P<0.01);总体上12.5和25 mg.kg-1剂量组作用较好,优于阳性对照药尼莫地平(P<0.05或P<0.01),用药72 h的脑梗死体积、bcl-2、bax mRNA与24 h比较有显著性差异(P<0.05或P<0.01)。另外,bcl-2和bax蛋白的表达情况与其基因表达趋势基本一致。以上结果提示,lactuside B可能通过上调bcl-2 mRNA增加其蛋白的表达和下调bax mRNA减少其蛋白的表达,在一定程度上发挥较好的抗脑缺血作用。
This study is to investigate the effect of the major chemical composition in rhizome of Pterocypsela elata,lactuside B,on expression of bcl-2,bax mRNA and their protein in rats＇ cerebral cortex after cerebral ischemia-reperfusion injury.First,middle cerebral artery ischemia-reperfusion injury model was established,and each group was treated with the corresponding medicines.Animals were separately sacrificed at 24 h and 72 h.The brain infarct volumes were detected by TTC dye,bcl-2 and bax mRNA expression was checked by RT-PCR,and the proteins of bcl-2 and bax were explored by two-step immunohistochemistry in cerebral cortex of rats.Lactuside B can reduce brain infarct volume of cerebral cortex of rats,increase the expression of bcl-2 mRNA and decrease that of bax mRNA.Moreover,the ratio of bcl-2 to bax mRNA is higher in 12.5 and 25 mg·kg-1 dose group,respectively,which is significantly different from that of model group（P 0.05 or P 0.01）.Generally,either 12.5 or 25 mg·kg-1 dose group is better than positive control medicine nimodipine（P 0.05 or P 0.01）.In addition,the expression of bcl-2 and bax protein is consistent with their gene expression.Infarct volume and the ratio of bcl-2 to bax mRNA expression are significantly different（P 0.05 or P 0.01） between 72 h and 24 h group.The results demonstrated that lactuside B could play a good role in resisting cerebral ischemia by upregulating the expression of bcl-2 mRNA and protein and downregulating that of bax mRNA and protein.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第11期1314-1320,共7页
Acta Pharmaceutica Sinica
基金
河南省教育厅自然科学研究项目(2009A310009)
国家自然科学基金资助项目(81172953)
