摘要
目的:通过检测外周血中生存素来判断是否能预测胃癌的复发与转移。方法:以反转录-聚合酶链式反应-酶联免疫测定(RT-PCR-ELISA)方法检测胃癌患者外周血中生存素mRNA的表达,分析其与临床病理因素之间的关系。对部分患者进行36个月随访,分析复发及转移时间与外周血中生存素mRNA的表达之间的关系。结果:53例胃癌外周血中32例(60.4%)生存素mRNA表达阳性。肿瘤大小>5cm及≤5cm生存素mRNA表达率分别为78.3%、46.0%。浸润深度达浆膜层者外周血生存素mRNA表达率74.2%,高于侵及黏膜下层及肌层的表达率(33.3%),淋巴结有转移者外周血表达率为75.0%,高于无淋巴结转移的表达率(29.4%)。通过随访研究发现外周血生存素阳性的患者复发及转移率明显高于阴性患者。结论:生存素mRNA在外周血的表达与肿瘤大小、浸润深度、淋巴结转移和临床分期均有明显相关性,但与肿瘤部位、分化程度无明显相关。并可用于提示患者复发与转移风险。外周血生存素的表达可作为评价胃癌生物学行为及预后的参考指标。
Objective： To investigate survivin expression in the peripheral blood of gastric cancer patients and whether it can predict the metastasis and recurrence of gastric carcinoma. Methods： Reverse transcriptase polymerase chain reaction and enzyme-linked irnmunosorbent assay （ RT-PCR-ELISA ） was used to assess the expression of survivin mRNA in the peripheral blood of patients with gastric cancer. The correlation between survivin expression and the clinicopathologic features was then calculated. During a follow-up period of 36 months, the correlation of time of metastasis and recurrence with survivin expression was analyzed. Results： The survivin mRNA expression rate in the peripheral blood of the 53 cases of patients with gastric cancer was 60.4% （ 32 cases ）. The expression rates for tumors 〉5 cm and≤ 5 cm were 78.3% and 46%, respectively. The expression rate among patients with infiltrated plasma membranes was 74.2%, which was higher than that in the remaining patients （ 33.3% ）. With lymph node metastasis, the expression rate was 75.0%, which was higher than in those without lymph node metastasis （ 29.4% ）. Conclusion： Survivin expression in the peripheral blood is correlated with the size, the presence of infiltration, lymph node metastasis, and clinical stages, but not with the primary tumor site or the degree of tumor differentiation. In addition, survivin expression can indicate that patients have a higher risk of metastasis and recurrence. Therefore, survivin expression in the peripheral blood might be a referenced indicator for evaluating the biological behavior and prognosis of gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2011年第20期1258-1261,共4页
Chinese Journal of Clinical Oncology
关键词
生存素
胃癌
反转录-聚合酶链式反应
酶联免疫测定
外周血
Survivin
Gastric cancer
Reverse transcriptase polymerase chain reaction
Enzyme-linked immunosorbent assay
Peripheral blood
