摘要
目的探讨不同作用时间下粒径为25nm的锐钛矿型二氧化钛(TiO2)颗粒对人肺腺癌A549细胞的毒性效应。方法将100μg/ml的纳米TiO2颗粒悬液与A549细胞在37℃、5%CO2的细胞培养箱中分别孵育1h、4h、8h和24h后,收集细胞。通过乳酸脱氢酶(LDH)检测实验检测纳米TiO2颗粒对细胞膜的损伤情况;通过ATP检测实验检测纳米TiO2颗粒对线粒体产生的影响;通过超氧化物歧化酶(SOD)活性检测实验检测纳米TiO2颗粒对细胞的氧化损伤情况;采用透射电子显微镜观察纳米TiO2颗粒引起细胞超微结构的变化;利用CCK-8法检测纳米TiO2颗粒对细胞存活率的影响。结果随着纳米TiO2颗粒作用时间的延长,细胞外液中LDH活性增强,细胞内ATP浓度降低,SOD活性降低,细胞存活率呈现明显下降(P<0.05),且细胞线粒体和内质网出现不同程度的肿胀和扩张。结论纳米TiO2颗粒体外能够引起肺腺癌细胞氧化损伤,抑制细胞生长,且对细胞的毒性效应存在时间依赖性。
Objective To investigate the toxic affects of 25nm TiO2nanoparticles in anatase crystal against human lung cancer cell line A549after different exposure periods.Methods A549 cells were incubated with 100μg/ml TiO2 nanoparticle suspension for 1h,4h,8hand 24hat 37℃ and 5%CO2.Then the damage degrees of cell membrane caused by TiO2 nanoparticle were assessed by LDH assay.ATP assay was used to evaluate the influence of TiO2nanoparticle on mitochondria.SOD activity test was used to detect the oxidative damage of the cells caused by TiO2 nanoparticles.The cellular ultrastructure changes of A549cells were observed by transmission electron microscopy.CCK-8 assay was performed to evaluate the viability of TiO2-treated cells after 1h,4h,8h and 24h exposure.Results With the prolongation of exposure time,the activity of extracellular LDH was increased and that of SOD was decreased;the concentration of intracellular ATP was reduced and the survival rate of cells was significantly decreased（P〈0.05）.The mitochondria and endoplasmic reticulum were swollen and expanded to different degrees.Conclusion TiO2 nanoparticles can result in oxidative damage and inhibit the growth of A549 cells,and the effects are in a time-dependent manner
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2011年第10期1091-1095,共5页
Academic Journal of Second Military Medical University
基金
国家重点基础研究发展计划(2006CB932505)
上海市科学技术委员会纳米专项(0752nm020)~~
