摘要
运用染色体原位显带方法,观察和分析26例食管癌患者和45例正常人淋巴细胞在常规培养条件下,自发和诱发(互隔交链孢霉提取物C12b3-2、MNNG)的染色体断裂热点(即频发断裂点)与脆性部位、肿瘤相关断裂点和癌基因位点的相关性。结果表明食管癌患者自发断裂点与脆性部位和肿瘤相关断裂点明显相关,提示自发断裂热点可能与该患者对肿瘤的易感性高有关;同时正常人的自发热点与脆性部位和肿瘤相关断裂点均不相关,可能与正常人对肿瘤易感性低有关。实验结果表明C12b3-2和MNNG诱发的新断点与肿瘤相关断点表现出明显相关,提示这两种致癌物的致癌作用可能与其对肿瘤相关断裂点的特异性有关。但无论是自发还是诱发断裂热点均不与癌基因位点相关。
26 patients with esophageal cancer and 45 normal individuals were studied. Chromosome banding technique in situ was used to localize breakpoints. Hot breakpoints, which were nonrandomy distributed, on chromosomes, contained spontaneous and induced(MNNG; C12b3-2,a kind of extract from Alternariol Alternata) breakpoints. The results indicated that (1)sponteneous hot breakpoints in the patients were significantly interelated with fragile sites (FS)and neoplasia-ass ociated breakpoints(NAB),but sponteneous breakpoints in normal individuals were not interrelated with FS and NAB; (2)C12b3-2 and MNNG induced hot points were significantly interrelated with NAB; (3)neither sponteneous nor induced hot brrakpoints were interrelated with oncogene sites.
出处
《遗传与疾病》
CSCD
北大核心
1990年第3期158-161,190,共4页
关键词
食管癌
癌基因
脆性部位
断裂热点
Esophageal cancer Chro mosal hot breakpoint Fragile sites Ne oplasia-associated Breakpoint oncogene
