摘要
以改进的去污剂微铺展技术制备普通小麦减数分裂联会复合体标本,并对联会复合体发育的全过程作了详细的电镜观察和描述。结果表明,小麦SC以多点式起始方式于偶线期开始形成;随SC的发育,新的SC形成和已有SC片断的延伸并存;此外,在同一核内不同二价体之间,染色体配对和SC形成并不同步;SC成熟于粗线期,而以破碎方式解体,消失于双线期。在偶线期还观察到由同祖配对形成的多价体,但在随后阶段中这些多价体消失。对Ph基因的可能作用机制作了分析和讨论。
A modified detergent spreading technique was used in ultrastructural study of the development of synaptonemal complex in wheat (Triticum aestivum). At zygotene, the SC formation initiated at multiple sites in each bivalent but the regions near telomeres were preferred. SCs were shortened with development of SCs. Initiation of new SC and extension of existing SC occured simultaneously, and pairing in different bivalents of a nucleus was not synchronised. SCs matured at pachytene. At diplotene, SCs broke down and disappeared. In addition, the homeologous multivalents have been observed in spreads of zygotene but these multivalems were ultimately resolved. The possible effect of Ph gene was also discussed.
关键词
小麦
联复合体
去污剂微铺展
Detergent spreading method, Synaptonemal complex, Wheat (Triticum ae-stivum)
