摘要
通过B.subtilis噬菌体PBSI转导,已将携带热稳定α-淀粉酶基因的质粒pAmy411引进了B.subtilis BF7658.转导频率为10_(-9)转导子/PFU。尽管pAmy 411的诲贝数在B.subtilis BF7658中较在B.subtilis AS 1.1176中高1倍,但其传代稳定性却较后者低。质粒携带的热稳定α-淀粉酶基因的表达水平在B.subtilis BF 7658中较在B.subtilisAS1.1176中高6倍。
The plasmid pAmy411 carrying thermostable α-amylase gene has been transduced into B. subtilis BF7658 by bacteriophage PBS1. The transduction frequency was 10-9 transductants per PFU. The stability of plasmid pAmy411 in B. subtilis BF7658 was lower than that in R. subtilis AS1.1176, whereas the copy numbers were double than that in B. subtilis AS1.1176. The expressive level of thermostable a-amylase gene was about 6 times higher than that in B. subtilis AS1.1176.
关键词
质粒转导
枯草芽孢杆菌
基因表达
Phage PBS1, Plasmid tra&sduction, Bacillus subnlis BF7658, Gene expression
