摘要
本文应用胰酶法在茶树的晚前期、前中期和早中期的每一染色体的全长上诱导出了清晰而丰富的G带带纹。染色体带纹的数目随染色体的浓缩程度而变化,同源染色体带纹的大小、分布及着色的深浅基本相似。作者认为植物G带的诱导与染色体处理技术及染色体所处的分裂时期密切相关。当胰酶处理超过了G带诱导的临界限度时,常可观察到染色体的大螺旋结构。本文讨论了在染色体前处理中a-溴萘的选用和甲醇-冰醋酸(3:1)的固定时间对G带诱导的影响以及G带的形成与染色体大螺旋结构之间的关系。
Clear and abundant G-banding at late prophase, prometaphase and early metaphase along the length of the tea chromosome has been induced by trypsin digestion. The number of G-bands varies in the degree of chromosomal contraction. Each homologous pair of chromosome resembles in the size, distribution and staining intensity of G-bands. It is suggested that the induction of G-bands for plant chromosomes is closely related to the treatment techniques and division phase of chromosomes. When trypsin treatment is beyond the critical limit for G-banding, the chromosomal macrocoil structures are usually revealed. The effects of pretreatment of cells with α-bromonaphthalene and fixation time of methanol-acetic acid (3:1) on the induction and the relationship between G-banding patterns and macrocoil structures are disscussed.
