摘要
提出了毋需柱前衍生化处理的高效液相色谱直接测定D型丝氨酸含量的方法。以pH1.0的高氯酸溶液为流动相,流量为0.3mL.min-1,Daicel CROWNPAK CR(+)色谱柱(4mm×150mm)进行分离,柱温0℃。采用170U紫外检测器,检测波长为200nm,D-丝氨酸及其主要杂质L-丝氨酸在10min内完全分离,分离度为2.09,其中D-丝氨酸的质量浓度在1.00～10.00g.L-1范围内呈线性。方法的检出限(3S/N)为0.20g.L-1,回收率在93.4%～95.7%之间,相对标准偏差(n=6)在3.2%～3.6%之间。
A direct method of HPLC without precoluran derivatization was proposed for determination of D- serine. Chromatographic column of Daicel CROWNPAK CR（＋） （4 mm× 150 mm） and mobile phase of pH 1.0 HCkO4 solution were used in the separation, with column temperature of 0 ℃ and flow-rate of 0. 3 mL · min^-1. 170U UV-detector with wavelength of 200 nm was adopted in the detection. Under the given conditions, the Dserine and its main impurity L-serine were completely separated from each other within 10 miru The resolution factor （Rs） attained to a value of 2.09. Linearity of D-serine was found in the range from 1.00 to 10. 00 g · L^-1. Detection limit （3S/N） found for the method was 0. 20 g · L^-1. Tests for recovery and precision were made by standard addition method, values of recovery and RSDPs （n= 6） obtained were in the ranges of 93.4%--95.7 % and 3. 2%--3. 6% respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2011年第10期1184-1185,1188,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
