摘要
目的探讨饱和氢生理盐水是否具有预防噪声性聋的作用。方法取清洁级白色健康、ABR阈值正常豚鼠15只,雌雄不限,体重250～300g。随机分成3组,空白对照组5只;生理盐水对照组5只;饱和氢生理盐水处理组5只,于噪声暴露前1小时给予腹腔注射饱和氢生理盐水,1ml/100g。给予脉冲噪声(压力峰值157dBSPL,脉宽0.25ms)连续暴露100次。于脉冲噪声暴露后即刻、第1天、第2天、第4天、第8天测听性脑干反应(auditory brainstem respons,ABR);第8天采用琥珀酸脱氢酶(Succinate Dehydrogenase,SDH)染色观察外毛细胞的形态学变化。结果噪声暴露后,饱和氢生理盐水处理组在各时间点的ABR阈值均明显低于对照组,有统计学意义(P<0.01);光学显微镜下可见空白对照组及生理盐水对照组动物耳蜗SDH几乎不着色,细胞肿胀或缺失,排列不规则;饱和氢生理盐水处理组SDH着色较深,存在散在片状无染色区,细胞缺失明显减少,排列较规则;耳蜗底回同一部位外毛细胞存活计数,空白对照组为45.17±12.15个,盐水对照组为44.50±10.02个,二者无明显差别;饱和氢生理盐水处理组分别为116.50±2.38个与对照组有统计学意义(P<0.05)。结论腹腔注射饱和氢生理盐水可拮抗强脉冲噪声引起的耳蜗损伤,能够对噪声性聋起到一定程度的预防作用。
Objective To study effects of saturated hydrogen saline in preventing noise-induced hearing loss. Methods Fifteen guinea pigs were randomly divided into 3 groups （5 each） to receive no treatment, treatment with normal saline or treatment with saturated hydrogen saline given intraperitoneally at 1 hour before noise exposure at 1 ml/ 100g. One hundred rounds of impulse noise （157 dB SPL peak） were delivered as noise exposure. Immediately alter ex- posure to impulse noise and on Days 1, 2, 4 and 8 following exposure, auditory brainstem response （ABR） thresholds were measured. Outer hair cell morphological changes and succinate dehydrogenase （SDH） activity were examined on Day 8 post-exposure. Results Immediately after noise exposure, ABR thresholds in saturated hydrogen saline treated animals were lower than the non-treated animals （P 〈 0.05）. Microscopy showed little SDH staining, cell swelling and irregular cell arrangement in the non-treated or normal saline treated animals. Whereas in the saturated hydrogen saline treated an- imals, there was deep SDH staining with significantly reduced cell loss and more regular cellular arrangement compared to
出处
《中华耳科学杂志》
CSCD
2011年第3期276-280,共5页
Chinese Journal of Otology
基金
国家自然科学基金30600700
30772413
全军医学科技十二五科研项目资助
主要在解放军耳研所完成
关键词
饱和氢生理盐水
噪声性聋
预防保护
修复
抗氧化
Saturated hydrogen saline
Noise-induced hearing loss
Protective effects
Repair
Antioxidan
