摘要
目的:探讨Bax-小干扰RNA(small interfering RNA,siRNA)和Bak-siRNA对小鼠胚胎发育及凋亡的效应。方法:通过对体外培养小鼠早期胚胎激光打孔,完成Bax-siRNA(Bax-siRNA组)、Bak-siRNA(Bak-siRNA组)和Bax-Bak-siRNA(Bax-Bak-siRNA联合组)的转染,并以阴性siRNA和纯水为阴性对照组和空白对照组,对确定转染效果的胚胎观察形态变化、生长发育状况及囊胚形成率,应用Hoechst 33342和碘化丙啶的方法分析各转染组小鼠早期胚胎细胞凋亡和增殖程度以及凋亡颗粒率。结果:鼠胚激光打孔后可成功转染Bax-siRNA、Bak-siRNA和Bax-Bak-siRNA。Bak-siRNA组和Bax-Bak-siRNA联合组鼠胚的囊胚形成率显著高于阴性对照组(P<0.01),而且凋亡颗粒率显著低于阴性对照组(P<0.01)。Bax-siRNA组的囊胚形成率和凋亡颗粒率与阴性对照组比较均无统计学差异(P>0.05)。结论:Bak-siRNA和Bax-Bak-siRNA可以改善小鼠胚胎的囊胚形成率,减少胚胎细胞的凋亡。
Objective： To investigate the effects of Bax-siRNA and Bak-siRNA by in vitro trasfection of cultured mouse embryos. Methods： Bax-siRNA, Bak-siRNA and Bax-Bak-siRNA were transfectedinto mouse embryos by zona drilling. Embryo morphology, development and blastocyst formation rate wereobserved. Early embryo apoptosis, proliferation / apoptotic ratio and apoptotic paticle rate were evaluated byHoechst 33342 and propidium iodide staining, respectively. Results： Transfection of Bak-siRNA and Bax-Bak-siRNA significantly increased the rate of blastocyst formation respectively （P〈0.01）. In both Bak-siRNA andBax-Bak-siRNA group, embryonic apoptosis was significantly reduced （P〈0.01）, while the group transfectedwith Bax-siRNA had no significant differences with that of the negative group （P〈0.05）. Conclusion： Both Bak-siRNA and Bax-Bak-siRNA could improve the blastocyst formation rate and reduce embryo apoptosis during early mouse embryo development.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2011年第10期647-651,670,共6页
Reproduction and Contraception
基金
国家自然科学基金项目
项目号:30872769
