摘要
荧光寿命显微成像(FLIM)技术是一种新颖的荧光成像技术,具有其他荧光成像方法无法替代的优异性能,是生物医学工程领域的研究热点。频域调制、门控探测和时间相关单光子计数(TCSPC)是FLIM的几种主要实现方法。综述了这些技术的原理、研究现状和已取得的部分成果,比较了这三种方法的时间分辨率和成像速度等参数的优劣。宽场FLIM更适用于延时成像和实时成像。荧光偏振各相异性成像和内窥镜FLIM技术都是FLIM技术很有前景的应用方向。
Fluorescence lifetime imaging microscopy （FLIM） technology has so many outstanding and unique advantages compared with other fluorescence imaging methods that it attracts more and more researchers inbiomedicine engineering field. Frequency domain modulation method and time domain methods including time-gated detection and time correlated single photon counting （TCSPC）, are the main ways to realize FLIM. Principles,current reaseach situation and achievements of these methods are presented as well as the comparison of their main performance parameters. Wide-field FLIM is more suitable for delay imaging. Fluorescence lifetime polarizationresolved imaging and endoscope FLIM are both promising research areas of FLIM.
出处
《激光与光电子学进展》
CSCD
北大核心
2011年第11期46-51,共6页
Laser & Optoelectronics Progress
关键词
测量
荧光寿命成像
单光子计数
门控探测
频域调制
measurement
fluorescence lifetime imaging microscopy
time-correlated single photon counting
timegated detection
frequency modulation
