摘要
合成和表征了一种叔丁基异氰酸酯全衍生化β-环糊精SBA-15硅胶手性固定相。在RP-HPLC条件下,采用5-cm短柱,分别实现了对包含β-受体阻滞剂普萘洛尔(propranolol)在内的8种含氮原子手性药物对映体的拆分,初步考察了流动相的组成、pH及流速对手性色谱分离的影响。上述药物对映体均在短时间(t<6 min)内得到了良好的分离。
a novel chiral stationary phase was successfully prepared by immobilization of （mono-6-ethylene diamino-6-deoxy-2,3,6-tert-butylisoeyanated） β-eyclodextrin onto the surface of a mesoporous silica gel （SBA- 15）. The resulting bonded silica stationary phase was characterized by instrumental analyses. Eight nitrogencontaining chiral enantiomers including four β-blocker drugs and others were successfully separated in RPHPLC, respectively. The effects of composition, buffer concentration and pH value in mobile phases on the chiral selectivity of β-blockers were also preliminarily investigated. The results showed that the short CD-based 5era-column exhibited rapid separation ability to the above drugs within 6 min, which indicates that the separation has great potential in fast enantioseparation. The established method is simple, fast, efficient and reproducible for the above chiral drugs.
出处
《分析试验室》
CAS
CSCD
北大核心
2011年第11期20-25,共6页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(21165012)
江西省自然科学基金(2010GZH0089)
江西省教育厅科技项目(GJJ11274)资助
