摘要
以米曲霉C-2为出发菌株,紫外诱变后,利用透明圈初筛,再分别以农业废弃物1%蔗渣-1%麸皮复合物、2%蔗渣、2%碱提蔗渣半纤维素和2%纤维素溶剂分馏法(CSLF)提取的蔗渣半纤维素为碳源摇瓶复筛,得到一株高产木聚糖酶的米曲霉菌株A73.结果表明:米曲霉A73在上述碳源培养基中,产木聚糖酶酶活力比出发菌株分别提高了281.15%,82.81%,56.09%和33.11%;CSLF蔗渣半纤维素诱导米曲霉C-2及其诱变菌株产酶能力较弱,明显低于其他3种诱导物.
Aspergillus oryzae C-2 was treated with ultraviolet irradiation. High-yield xylanase mutant A73 was obtained after primary transparent circle screening and secondary screening in which 4 different carbon sources-1% wheat bran-1% sugarcane bagasse, 2% sugarcane bagasse, 2% alkali pretreatment hemi-cellulose and 2% CSLF （cellulose solvent-based lignocellulose ffactionation） pretreatment hemi-cellulose were used as fermentation substrates. The xylanase activity of Aspergillus oryzae A73 improved 281.15%, 82. 81%, 56.09% and 33. 11% compared with the original strain on respective carbon source, but the xylanase activity induced by CSLF pretreaiment hemi-cellulose was apparently lower than that of other substrates.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2011年第6期663-667,共5页
Journal of Huaqiao University(Natural Science)
基金
福建省自然科学基金资助项目(D0810015)
教育部留学回国人员科研启动项目(2010)
福建省高等学校新世纪优秀人才支持计划项目(07FJRC03)
关键词
木聚糖酶
米曲霉
紫外诱变
纤维素溶剂木质纤维素分馏法
半纤维素
xylanase
Aspergillus oryzae
ultraviolet irradiation
cellulose solvent-based lignocellulose fractionation
hemi-eellulose
