摘要
目的建立高效液相色谱法（HPLC）测定注射用阿魏酸钠含量的方法。方法色谱柱：Waters X Terra RP18（4．6mm×250mm，5um），流动相为甲醇-水（62：38），流速为1ml·min-1，检测波长为310nm。灵敏度2．000AUFS，柱温30℃，进样20ul。结果阿魏酸钠在44．08～220．4ug·ml-1范围内线性关系良好（r=0．9997），平均回收率98．79％，相对标准偏差（RSD）为1．61％。结论本方法简便、准确、专属性强，可用于阿魏酸钠含量的测定。
Objective To determine the content of sodium ferulate for injection by HPLC. Methods HPLC meth- od was used with Waters X Terra RPI8 column. The mobile phase was methanol -water （62：38）. The flow rate was 1 ml · min-1. The detective wavelength was 310 nm. The column temperature was 30℃ . Result The linear range of the sodium ferulate was within 44.08 - 220.4 ug · ml-1 （ r = 0. 9997） , the recovery rate was 98.79% with RSD of 1.61%. Conclusion This method is accurate and sensitive to determine the content of the sodium ferulate for injection.
出处
《徐州医学院学报》
CAS
2011年第10期708-709,共2页
Acta Academiae Medicinae Xuzhou
关键词
高效液相色谱法
阿魏酸钠
含量测定
high - performance liquid chromatography
sodium ferulate
content determination
