摘要
根据实验室测序的大豆线粒体基因组片段设计线粒体细胞色素b(Cytochrome,cob)基因特异引物,PCR扩增获得1 173 bp大豆(JLGM-1B)线粒体cob基因保守区序列。经序列分析,该基因编码391个氨基酸,A+T含量58.1%,G+C含量41.9%。Southern杂交表明cob基因在栽培大豆线粒体基因组中至少有2个拷贝。氨基酸序列及系统进化树分析表明,大豆与其它植物的cob基因具有较高的同源性,与双子叶作物同源性高达98%,其分类地位与传统系统分类地位相吻合,说明cob基因具有较高的保守性,可以作为分子系统分类学潜在的遗传分化标记。
In this study,mitochondrial DNA uncompleted sequence was used to design special prime of mitochondrial cytochrome b gene（cob）and obtained conservation domain of soybean cob gene by PCR amplification.The results showed that cob gene was consisted by 1 173 bp which encoded 391 amino acids,with 58.1% A＋T and 41.9% G＋C content.Southern blot analysis revealed that more than 2 cob gene copies were found in G.max mitochondrial genome.Amino acid sequence and phylogenetic analysis showed that cob was most similar to the dicotyledon,with 98% identity at the nucleotide level.In conclusion,cob gene is a potential hereditary marker for phyletic classification basing on its highly conservative property.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第5期738-742,共5页
Soybean Science
基金
国家转基因重大专项资助项目(2008ZX08004-004)
国家自然科学青年基金资助项目(31000141)
国家高技术研究发展计划重点资助项目(2006AA10Z120
2011AA10A105)
关键词
大豆
线粒体
细胞色素B
Soybean
Mitochondrial
Cytochromeb
