摘要
目的:评价重组人胰岛素样生长因子1(recombinant Insulin-like growth factor-1,rhIGF-1)对新生儿缺氧缺血性脑病(hypoxic ischemic encephalopathy,HIE)模型大鼠的认知功能、海马细胞形态学变化及Akt1 mRNA表达量的影响,为临床上治疗HIE提供理论依据。方法:选取7日龄SD大鼠15只,随机分为模型组、治疗组和对照组,运用缺血灌注和缺氧处理模型组和治疗组大鼠,建立HIE模型。治疗组给予rhIGF-1鼻吸处理2次(间隔1 h)。15 d后,用Morris水迷宫观察大鼠逃避潜伏时间(s),观察海马区CA1区细胞数量,运用RT-PCR方法检测Akt1表达量变化。结果:与模型组大鼠相比,治疗组大鼠学习记忆能力明显提高,海马CA1区细胞数量明显增多,海马区Akt1 mRNA表达明显增多(F检验,P<0.05),模型组上述指标均低于对照组(F检验,P<0.05)。结论:rhIGF-1可以明显改善HIE大鼠认知功能,增加海马区Akt1 mRNA表达,有明显的脑保护作用。
Objective：To evaluate the effect of recombinant human insulin-like growth factor-1（rhIGF-1） on cognitive function in rats with hypoxic-ischemic encephalopathy（HIE） and to provide evidences for treatment.Methods：15 SD rats of 7-day-old were randomly divided into model group,treatment group and control group.Established HIE model,model group and treatment group rats were treated with monolateral carotid ligation and hypoxia;at the same time treatment group received rhIGF-1 by intranasal administration（2 times,interval 1 hour）.After 15 days,the cognitive function of rats were measured with Morris water maze test,and number of cells,Akt1 mRNA expression in hippocampal CA1 sector were tested.Results：Compared with model group,the cognitive function of escape latency in the maze test were increased significantly（P0.05）;and number of cells,Akt1 mRNA expression in hippocampal CA1 sector were also increased in treatment group（P0.05）.Conclusion：RhIGF-1 can significantly improve cognitive function in rats with HIE.,enhances the Akt1 mRNA expression in hippocampal CA1 sector,and have protective effects on brain.
出处
《现代临床医学》
2011年第3期169-171,共3页
Journal of Modern Clinical Medicine
