摘要
目的通过比较牙周炎发展不同阶段唾液中白细胞蛋白酶抑制因子（secretory leukocyte protease inhibitor，SLPI）浓度和弹性蛋白酶（elastase，EA）活性，分析机体在炎症发展不同阶段分泌的炎症相关蛋白在致炎、抗炎方面是否有差异，以及SLPI是否可以作为监测牙周炎发展和判断预后的实验室指标。方法采集86例受试者（分成健康对照组17例、牙龈炎组18例，中度慢性牙周炎组31例、重度慢性牙周炎组20例）的非刺激性全唾液，用酶联免疫吸附测定法检测SLPI质量浓度，底物法检测EA活性；86名受试者中有15例中度或重度慢性牙周炎患者仅接受了牙周机械治疗（洁治、刮治和根面平整），在治疗前和治疗后4周均进行了唾液采集和临床指标的记录。结果中度和重度慢性牙周炎组EA活性[分别为0．077（0．060）和0．077（0．489）]显著高于健康对照组[0．058（0．028）]和牙龈炎组[0．058（0．024）]（P〈0．05），而SLPI质量浓度在4个组中差异均无统计学意义[分别为1．031（1．300）、1．295（1．162）、1．554（1．774）和1．944（4．734）汕g／L，P〉0．05]。SLPI与年龄呈负相关关系（r=-0．301，P〈0．05），与EA和临床指标之间无显著相关性。15例慢性牙周炎患者治疗前，唾液中SLPI质量浓度和EA活性分别是2．031（2．449）μg／L和0．075（0．118），牙周机械治疗4周后分别降至1．405（0．659）μg/L和0．055（0．028），与治疗前相比差异均有统计学意义（P〈0．05）。结论基础治疗后唾液中SLPI质量浓度和EA活性的变化可能反映牙周组织炎症的消退，唾液中SLPI在牙周炎症发展不同阶段无显著变化，SLPI的生成是否受到年龄的影响还需要进一步的研究证实。
Objective To investigate the change of secretory leukocyte protease inhibitor （SLPI） and elastase（EA） in the different stages of periodontal inflammation and to evaluate the possibility of the two proteins as saliva markers reflecting overall periodontal health status. Methods Unstimulated whole saliva were collected from 86 subjects （divided into 4 groups as healthy, gingivitis, moderate periodontitis and severe periodontitis）. Fifteen patients with moderate or severe periodontitis were only given scaling and root planning （SRP）. Whole saliva was collected and clinical patameters were recorded at baseline and four weeks after the treatment. SLPI concentrations were determined with enzyme linked immunosorbent assay （ELISA） systems, while EA with low-molecular-weight substrate reaction. Results There were no statistical differences of SLPI concentrations among four groups （ P 〉 0. 05 ）. However, EA activities in moderate periodontitis and severe periodontitis groups [ 0. 077 （ 0. 060 ） and 0. 077 （ 0. 489 ） ] were higher than in healthy and gingivitis group [ 0. 058 （0. 028 ） and 0. 058 （0. 024） ] （ P 〈 0. 05 ）. SLPI only showed a weak negative correlation with age （r = -0. 301 ,P 〈0. 05） , rather than with EA or clinical parameters. In 15 patients with chronic periodontitis the mean concentration of SLPI and EA activity was 2. 031 （2. 449 ）μg/L and 0. 075 （ 0. 118 ） , and both decreased significantly to 1. 405 （ 0. 659 ） μg/L and 0. 055 （0. 028） respectively 4 weeks after SRP. Conclusions After SRP, the decrease of SLPI concentration and EA activity in saliva may reflect the periodontal inflammation subsiding. SLPI in saliva was not correlated with the development of periodontal inflammation.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2011年第10期581-585,共5页
Chinese Journal of Stomatology
关键词
胰弹性蛋白酶
牙周炎
唾液
白细胞蛋白酶抑制因子
Pancreatic, elastase
Periodontitis
Saliva
Secretory leukocyte protease inhibitor
