摘要
目的:研究不同海拔:青海都兰(海拔3 400m)、四川绵阳(海拔400m)汉族人群第21号染色体D21S1432、D21S1435、D21S1270、D21S1440、D21S1446、GATA24H09、ATA42C09、GATA129D11等8个STR位点的遗传多态性。方法:运用PCR扩增、6%变性聚丙烯酰胺凝胶电泳结合银染技术对不同海拔汉族人群无血缘个体进行遗传多态性研究。结果:青海世居汉族有110个基因型,频率分布在0.028 69～0.067 61之间。四川汉族有113个基因型,频率分布在0.025 99～0.067 55之间,8个STR基因座基因型分布均符合Hardy-Weinberg平衡。累积多态信息量青海为:0.685 6,四川为:0.631 8。累积杂合度为青海为:0.723 9,四川为:0.736 7。累积个体识别率(discrimination power,DP)青海为0.999 7,四川:0.996 5。结论:不同海拔汉族8个位点STR基因座的多态性数据显示有其自身的STR等位基因分布特征,两者等位基因频率比较无显著性差异(P>0.05)。
Objective：To analyze genetic polymorphism of STR（D21S1432,D21S1435,D21S1270,D21S1440,D21S1446,GATA24H09,ATA42C09,GATA129D1）located in number 21 chromosome from Han nationality population in Qinghai（3 400m） or Sichuan（400m）.Methods：The study was consisted of 32 Han native population and healthy mutual unrelated participants who were recruited from Dulan,Qinghai,and 34 similar nationality and general condition from Mianyang,Sichuan.All participants＇ genetic polymorphism was assessed by PCR and 6% denaturing PAGE and silver staining.Results：Qinghai participants had 110 genotypes.The genotype distribution was in accordance with Hardy-Weinberg equilibrium and its frequencies rang from 0.0286 9 to 0.0676 1.Sichuan participants had 113 genotypes and frequencies rang from 0.025 99 to 0.067 55.In Qinghai people,the calculated PIC,calculated HET and calculated DP was 0.685 6,0.723 9 and 0.999 7,respectively;0.631 8,0.736 7 and 0.996 5,respectively for Sichuan people.Conclusions：There is different characteristic in the eight STR loci distribution in Han population at different geographic altitude,but the allele frequency does not differ significantly between Qinghai and Sichuan population（P0.05）.
出处
《高原医学杂志》
CAS
2011年第2期14-17,共4页
Journal of High Altitude Medicine
基金
青海省自然科学基金课题Y36-2002-36
