摘要
目的 探讨颅内海绵状血管瘤的病理超微结构特征及其血管壁内促血管生成素-1（Ang-1）、CD68、基质金属蛋白酶-9（MMP-9）的表达情况与其反复出血的关系。方法选取颅内海绵状血管瘤标本35例、肝海绵状血管瘤标本35例及正常脑组织血管标本4例，采用光镜及电镜观察其血管超微结构特征，免疫组化染色检测其血管壁内Ang-1、CD68、MMP-9的表达水平。结果颅内海绵状血管瘤血管壁薄，仅有单层内皮细胞和较薄的外膜，缺乏肌层和弹力层．细胞间可见明显的间隙连接，基底膜模糊显示不清，血管周边有大量电子密度高的沉积物。CD68及MMP一9在颅内海绵状血管瘤中表达明显增高，与对照组相比差异有统计学意义（P〈0．05）。CD68与MMP-9表达之间秩和相关分析示两者呈高度正相关（r=0．870，P=-0．000）。Ang-1在颅内海绵状血管瘤中表达较低，与对照组相比差异无统计学意义（P〉0．05）。结论颅内海绵状血管瘤的血管在结构和功能上的缺陷和不稳定是其出血的前提条件，CD68、MMP-9的高表达与其出血密切相关。
Objective To investigate the relation between recurrent hemorrhage and both ultrastructural features of vascular and expressions of angiopoietin 1 （Ang-1）, CD68 and matrix metalloproteinase-9 （MMP-9） in intracranial cavernous hemangioma. Methods Thirty-five intracranial cavernous hemangioma samples, 35 hepatic cavernous malformation samples and 4 normal brain tissues were chosen in our study; the ultrastructural features of vascular were observed with optical microscope and electron microscope; immunohistochemical methods were used to analyze the expressions of Ang-1, CD68 and MMP-9. Results The vascular wall was very thin in intracranial cavernous hemangioma samples, only with thin monolayer of endothelial cells and adventitia, but without basal lamina and elastic layer; gap junctions were obviously observed between endothelial cells; basement membrane showed unintelligible, and a large number of high electron density of sediment was presented at the surrounding blood vessels. Tissues from intracranial cavernous hemangioma obviously expressed higher levels of CD68 and MMP-9 than the normal brain tissues （P〈0.05）; Rank correlation analysis indicated that the expressions of CD68 and MMP-9 had a high degree of positive correlation （r-0.870, P=0.000）. Tissues from intracranial cavernous hemangioma expressed less Ang-1 than normal brain tissues without significant difference （P〉0.05）. Conclusion The vascular structural and functional defection and instability in patients with cavernous hemangioma provide favorable conditions for the happaning of recurrent hemorrhage; and high expressions of CD68 and MMP-9 are closely related with recurrent hemorrhage.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2011年第10期1019-1022,共4页
Chinese Journal of Neuromedicine
