摘要
采用气相色谱-质谱联用技术对小叶红叶藤挥发油的化学成分进行分析,并用DPPH·法、ABTS·+法和铁氰化钾还原法对其抗氧化能力进行研究。结果表明:从小叶红叶藤挥发油分离出221个色谱峰,共鉴定64个化合物,占挥发油总量的95·72%,主要成分为dl-薄荷酮(53·43%)、(E)-薄荷酮(14·20%)、5-甲基-2-异丙基-3-环己烯-1-酮(9·87%)、β-萜品烯(8·16%)、β-蒎烯(1·54%)、α-蒎烯(1·18%)等。抗氧化实验中,不同浓度的挥发油对DPPH和ABTS+自由基有较好的清除能力,最高清除率分别达到62·25%和68·11%,但其还原能力较弱,抗氧化能力均具有明显的量-效关系。
The constituents of the essential oils of Rourea microphylla from Guangxi were separated and identified by GC-MS. The scavenging activities on DPPH radical,ABTS ＋ radical and reducing power were detected by UVVis spectrophotometry. 221 compounds were isolated and 64 compounds were identified that composed about 95.72% of the total essential oils. The principal chemical constituents of the essential oils were d/-Menthone （ 53.43 %）, （ E）-Menthone （ 14. 20 % ）, 2-isopropyl-5-methyl-3-Cyclohexen-l-one （ 9. 87 % ）, fl-Terpinene （ 8. 16 % ）, fl- Pinene（1.54%）, a-Pinene （1.18%）, et al. These oils possessed good scavenging activities on both DPPH and ABTS ＋ radical,the scavenging rate reaching to 62.25% and 68. 11%, respectively, but showed low activities on reducing power. All antioxidant activity showed a concentrationeffect relationship.
出处
《广西植物》
CAS
CSCD
北大核心
2011年第5期706-710,共5页
Guihaia
