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CFTR及SPINK1蛋白在慢性胰腺炎及胰腺癌中的表达及其意义 被引量：7

Expressions and signification of CFTR and SPINK1 in chronic pancreatitis and chronic pancreatitis
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摘要 目的探讨囊性纤维化跨膜转运调节因子(cystic fibrosis transmembrane conductance regulator,CFTR)及丝氨酸蛋白酶抑制剂Kazal 1型(Serine protease inhibitor Kazal type 1,SPINK1)在慢性胰腺炎及胰腺癌发病中的表达及其意义,为慢性胰腺炎及胰腺癌的早期诊断及预防提供实验依据,进而从基因水平上另辟新径。方法收集吉林大学第一医院病理科及白求恩医学院病理系存档的正常胰腺组织10例,慢性胰腺炎20例,胰腺癌30例。采用免疫组织化学染色方法(SP法)分别观察了石蜡标本中CFTR及SPINK1蛋白的表达情况。结果 CFTR及SPINK1蛋白在正常胰腺组织中呈强表达,阳性表达率均为100%(10/10);在慢性胰腺炎中表达均下降,阳性表达率分别为50%(10/20)和55%(11/20),与正常胰腺组相比,具有统计学意义(P<0.05);胰腺癌中二者的表达强度明显降低,阳性率分别为10%(3/30)和6.7%(2/30);与正常胰腺组及慢性胰腺炎组比较均具有明显差异(P<0.05)。结论 CFTR及SPINK1蛋白的异常表达与慢性胰腺炎和胰腺癌的发生、发展有关,二者的表达异常可能分别或协同参与了慢性胰腺炎及胰腺癌的发病过程。 Objective To investigate the expression and the role of cystic fibrosis transmembrane conductance regulator（CFTR） and Serine protease inhibitor Kazal type 1（SPINK1）in chronic pancreatitis and pancreatic cancer.Provide exprerimental evidence and opened up new avenues for the prevention and treatment of pancreatitis and pancreatic cancer from gene level.Methods Collecte paraffin specimen of normal pancreas,chronic pancreatitis and pancreatic cancer in Department of Pathology,First Hospital of Jilin University and Department of Anatomy,School of Basic Medical Pathology,Number of cases were repectively 10 cases,20 cases,30 cases.Observed the expression of the CFTR and SPINK1 in paraffin protein repectively with immunochemistric staining.Results CFTR and SPINK1 protein in normal pancreatic tissue showed strong expression,the positive expression rate is 100%（10/10）;Decreased expression in chronic pancreatitis,the positive expression rate is 50%（10/20） and 55%（11/20） respectively,compared with the normal pancreas,statistically significant（P0.05）;.The expression of CFTR and SPINK1 was significantly reduced in pancreatic cancer,the positive expression rate is 10%（3/30）and 6.7%（2/30）respectively,compared with the the normal pancreas and chronic pancreatitis group,there were significant differences（P0.05）.Conclusion Abnormal expression of CFTR and SPINK1 may play an important role in patients with chronic pancreatitis to pancreatic cancer and its transformation process.
作者 张敏 王银萍 倪劲松 薛世泉 邹亚彬 张丽红
机构地区 吉林大学白求恩医学院病理生物学教育部重点实验室 吉林大学第一临床医院病理科
出处 《中国实验诊断学》 北大核心 2011年第10期1625-1628,共4页 Chinese Journal of Laboratory Diagnosis
基金 吉林省科技厅国际合作项目(20050705-4)
关键词 CFTR SPINK1 慢性胰腺炎 胰腺癌 CFTR SPINK1 chronic pancreatitis pancreatic cancer
分类号 R576 [医药卫生—消化系统] R735.9 [医药卫生—肿瘤]
  • 相关文献

参考文献10

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二级参考文献34

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共引文献3

同被引文献29

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引证文献7

二级引证文献10

中国实验诊断学
2011年 第10期

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