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LAMP(环介导等温扩增)的原理及在寄生虫检测上的应用 被引量:7

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摘要 2000年,Notomi等[1]建立了一种快速、简单、灵敏、特异并且低成本的核酸扩增方法——环介导等温扩增法(LAMP),这种新颖的核酸扩增方法具有许多优势[2-4]:(1)扩增效率极高,60min内扩增产物可达到靶基因的109~1010倍;(2)操作简单,
出处 《中国人兽共患病学报》 CAS CSCD 北大核心 2011年第10期935-939,共5页 Chinese Journal of Zoonoses
基金 河南省科技厅重点科技攻关项目(92102110138)资助
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  • 1Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Research,2000,28(12):63.
  • 2Thekisoe OM,Inoue N,Kuboki N,et al.Evaluation of loopmediated isothermal amplification (LAMP),PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs[J].Veterinary Parasitology,2005,130(3-4):327-330.
  • 3Soliman H,EI-Matbouli M.Reverse transcription loop-mediated isothermal amplification(RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus(VHS)[J].Veterinary Microbiology,2006,114(3-4):205-213.
  • 4潘树德,刘宝山,刘金铃,李学俭,边连全,付静涛.环介导等温扩增技术及其在病原检测中的应用[J].动物医学进展,2009,30(2):74-77. 被引量:9
  • 5Nagamine K,Hase T,Notomi T.Accelerated reaction by loopmediated isothermal amplification using loop primers[J].Molecular and Cellular Probes,2002,16(3):223-229.
  • 6Thekisoe OM,Kuboki N,Nambota A,et al.Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis[J].Acta Tropica,2007,102(3):182-189.
  • 7Njiru ZK,Mikosza AS,Matovu E,et al.African trypanosomiasis:Sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA[J].International Journal for Parasitology,2008,38(5):589-599.
  • 8Karais P,Thekisoe O,Kioiptsi K,et al.Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples[J].Applied and Environmental Microbiology,2007,73(17):5660-5662.
  • 9Bakheit MA,Torra D,Plaomino LA,et al.Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing[J].Veterinary Parasitology.2008,158(1-2):11-22.
  • 10袁忠英,沈玉娟,曹建平,周何军,卢潍媛,陈勤,倪奕昌,汤林华.环介导等温扩增技术检测牛源隐孢子虫的研究[J].国际医学寄生虫病杂志,2009,36(3):144-146. 被引量:12

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