摘要
目的:探讨子宫内膜腺癌组织中HER-2/neu、COX-2、PGE_2、P450arom4种生物学指标的表达,分析这4种生物学指标表达强度之间相关性及与临床病理因素的相关性。方法:应用逆转录-聚合酶链反应(RT-PCR)技术及酶联免疫吸附试验(ELISA)检测HER-2/neu、COX-2、P450arom及PGE_2在正常子宫内膜、子宫内膜不典型增生和子宫内膜腺癌组织中的表达情况。结果:HER-2/neu、COX-2、P450arom及PGE_2在癌组织中表达高于非癌组织,有显著性差异(P<0.01)。癌组织中HER-2/neu与COX-2、P450arom表达强度呈正相关(r值分别为0.931,0.934,P<0.05),COX-2与P450arom表达强度亦呈正相关(r=0.908,P<0.05)。COX-2mRNA和PGE_2表达强度变化呈正相关(r=0.552,P<0.05)。结论:HER-2/neu、COX-2、P450arom及PGE_2可能存在协同作用,共同促进子宫内膜腺癌的发生发展。
Objective： To discuss the expression of four biological parameters, namely, HER-2/neu, COX-2, PGE2, andP450arom, in endometrial carcinoma as well as to analyze their relationship in the expression patterns and all kinds of clinicopathologic factors. Methods： Reverse transcription polymerase chain reaction and ELISA were used to detect the expression of HER-2/neu, COX-2, P450arom, and PGE2 in normal endometrium, endometrial hyperplasia, and endometrial carcinoma. Results： The expression levels of HER-2/neu, COX-2, P450arom, and PGE2 were significantly higher in tumorous tissue than in nontumorous tissue （ P 〈 0.01 ）. There was a positive correlation among the expression levels of HER-2/neu, COX-2, and P450arom in the tumor （ r = 0.931, r = 0.934, P 〈 0.05 ）. COX-2 expression was positively correlated with that of P450arom in tumorous tissue （ r =0.908, P 〈 0.05 ）. There was also a correlation between the expression of COX-2 mRNA and PGE2 in the tumor （ r = 0.552, P 〈0.05 ）. Conclusion： HER-2/neu, COX-2, P450arom, and PGE2 may have a synergistic effect on the oncogenesis and progression of endometrial adenocarcinoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2011年第19期1183-1186,1195,共5页
Chinese Journal of Clinical Oncology
基金
辽宁省科学技术厅基金(编号:2007225002-3)
辽宁省科技厅百千万人才工程项目(编号:2010921071)资助~~
