摘要
以自然老化桑种子为材料,用2种不同浓度的化学试剂:赤霉素(GA3)、抗坏血酸(ASA)浸种处理,对桑种子的发芽力、α-淀粉酶活力以及幼叶中可溶性蛋白含量、可溶性糖含量和丙二醛(MDA)含量进行了分析。结果表明,2种试剂对桑种子萌发均有一定的促进作用,而且不同试剂对种子萌发的影响与使用的浓度密切相关。ASA处理以500 mg/L效果最好,GA3处理以50 mg/L效果最好。其中500 mg/L ASA处理种子发芽率、发芽指数、α-淀粉酶活力、桑幼叶中可溶性蛋白含量、可溶性糖含量比对照分别提高了13.87%、16.18%、55.68%、17.20%和30.53%;50 mg/L GA3处理分别比对照提高了4.63%、14.72%、28.41%、12.45%和12.98%;丙二醛含量500 mg/L ASA处理和50 mg/L GA3处理分别比对照下降了56.32%和37.62%。500 mg/L ASA处理下,除α-淀粉酶活力外,其余指标各处理间差异均达显著或极显著水平;50 mg/L GA3处理则表现为除发芽率和可溶性蛋白含量外,其余指标各处理间均达到显著或极显著水平。综合分析可知,500 mg/L ASA的处理效果比50 mg/L GA3好。
In order to explore the methods of improving the vigors of aging seeds, the mulberry seeds naturally aged were soaked with different concentrations of Gibberellin （ GA3 ） and aseorbic acid （ASA） for 24 hours. The seed germination vitalities, the α-diastatic activity and the malondialdehyde （MDA） in the leaves of and the contents of soluble protein, the soluble sugar seedlings were detected. The results showed that the two chemical reagent had some role in promoting mulberry seed germination ability, and their effects showed the relationship with the applied concentrations. The best effects were the treatments of 500 mg/L ASA and 50 mg/L GA3 , compared with the control, the germination rate, the germination index, the α-diastatic activity, contents of soluble protein and soluble sugar were respectively increased by 13.87%, 16. 18%, 55.68%, 17.20% and 30. 53%, while under the treatments of 50 mg/L GA3, respectively increased by 4. 63% , 14.72% ,28.41% ,12.45% and 12.98%. The MDA contents reduced 56.32% with treatment of 500 mg/L ASA, and 37.62% with treatment of 50 mg/L. In addition, the results also showed that between the treatments of 500 mg/L ASA,in addition to α-diastatic activity, the other physiological indexes had significant or highly significant differences, while between the treatments of 20 mg/L GA3 , except germination ratio and soluble protein content, the other physiological indexes also had significant or highly significant differences. Based on a comprehensive analysis of the effects on the germination ability and the vigor of aging mulberry seeds, the effects of 500 mg/L ASA was better than the effects of 50 mg/L GA3.
出处
《种子》
CSCD
北大核心
2011年第10期8-11,共4页
Seed
基金
国家蚕桑产业技术体系建设项目(nycytx-27-gw3032008)
