摘要
目的探讨细胞凋亡及半胱氨酸蛋白酶-3(caspase-3)在慢性阻塞性肺疾病(COPD)中的作用。方法分别对吸烟COPD患者24例、不吸烟COPD患者19例、健康志愿者15例收集支气管肺泡灌洗液,应用TUNEL法检测细胞凋亡,免疫细胞化学(SP)法检测caspase-3表达。结果吸烟COPD组和不吸烟COPD组的凋亡指数分别为(31.22±8.45)、(29.30±7.11),与对照组比较,有显著性差异;吸烟COPD组和不吸烟COPD组caspase-3表达积分分别为(5.74±1.83)、(5.39±1.26),与对照组比较,有显著性差异;COPD患者细胞凋亡及caspase-3表达与吸烟无关。结论细胞凋亡可能在COPD发生发展中起重要作用,caspase-3与COPD密切相关。
Objective To explore the role of the cell apoptosis and caspase-3 in chronic obstructive pulmonary diseases（COPD）.Methods The bronchoalveolar lavage fluid of 24 cases of smoking patients suffering from COPD,19 cases of non-smoking patients suffering from COPD and 24 cases of healthy volunteers were respectively collected.TUNEL was used to test the apoptosis and immunocytochemistry was applied to examine the expression of caspase-3 protein.Results The apoptotic index of smoker and non-smoker suffering from COPD were（31.22±8.45） and（29.30±7.11） higher than those in healthy volunteers.The caspase-3 protein expression of smoker and non-smoker suffering from COPD were（5.74±1.83） and（5.39±1.26） higher than those in healthy volunteers.The expression of caspase-3 protein and apoptosis in the COPD patients had nothing to do with smoking.Conclusion Apoptosis probably plays an important role in the development of chronic obstructive pulmonary diseases,and caspase-3 has a close relation to chronic obstructive pulmonary diseases.
出处
《实用临床医药杂志》
CAS
2011年第17期30-32,36,共4页
Journal of Clinical Medicine in Practice
基金
桂林医学院科研资助项目(KY2008001)
