摘要
预测与分析HP-PRRSV BJBLZ株序列特点,为进一步该毒株的研究做准备。利用分子生物学软件及服务器,对HP-PRRSV BJBLZ株全序列及各结构蛋白的生化特性、溶解性和核定位信号等进行了分析,结果显示HP-PRRSV BJBLZ株至少可划分12个非结构蛋白和6个结构蛋白;同源性比较显示GP3和GP5蛋白变异性高,M和N蛋白保守。GP3、GP5和M蛋白糖基化位点与参考株有变化,GP2、GP4和N蛋白糖基化位点与所有参考株一致。服务器预测显示,N蛋白的碱性氨基酸比例明显高于其他蛋白,总平均亲水值明显低于其他蛋白;GP2、GP3和GP4蛋白不稳定,GP5、M和N蛋白稳定。6个结构蛋白预测不溶性概率均占66%以上,GP3不溶性概率最高。可以利用以上一些不同于以往各代表株的特点,为PRRS的诊断和防治进行进一步的研究。
The properties of HP-PRRSV BJBLZ sequence were predicted and analyzed,which could provide reference prepare for further study of this strain.Molecular biology software and server were used to analyze the complete sequence of HP-PRRSV BJBLZ strain,the biochemistry properties,solubility of various stuctural proteins and nuclear location signal.The results showed that there are 12 non-structural and 6 structural protein.When we compare different protein homology,we found that GP3 and GP5 are high mutant,while M and N are conservative.The Protein Glycosylation Site of GP3,GP5 and M are different from the reference strains,but GP2,GP4 and N are the same.Server showed the ratio of Alkaline Amino acid of N is higher than others,and the total average hydrophilic value obviously is lower than other proteins.GP2,GP3 and GP4 are unstable,but GP5,M and N.Prediction of 6 structural protein insoluble probability are accounted for more than 66 percent,GP3 is highest.The properties of above strains could pave the way for the diagnosis and treatment of PRRS.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第10期185-190,198,共7页
Biotechnology Bulletin
基金
北京市农林科学院青年基金项目(2007020122)
新疆生产建设兵团博士资金项目(2011BB022)
关键词
高致病性
猪繁殖与呼吸障碍综合征
序列分析
BJBLZ株
Highly pathogenicity Porcine reproductive and respiratory syndrome（PRRS） Sequence analysis BJBLZ strain
