摘要
背景:Hedgehog作为骨发育中一种重要调控因子,近几年其在骨生长中作用机制的研究备受关注。目的:介绍Hedgehog在软骨组织和骨组织发育中的作用机制及其与骨疾病的关系,从而分析Hedgehog信号通路与骨发育的研究现状及发展趋势。方法:应用计算机检索中国期刊全文数据库和PubMed数据库,以"Hedgehog,骨发育,间充质干细胞,软骨,成骨,骨缺陷"和"Hedgehog,bone development,mesenchymal stem cells,cartilage,osteogenesis,bone defects"为检索词。最终共纳入31篇文献进行综述。结果与结论:Hedgehog信号与骨发育各阶段密切相关,包括间充质细胞向骨细胞分化,软骨组织和骨组织形成等各方面。其信号通路传导异常会导致各种骨畸形或骨缺陷。但是Hedgehog信号在骨发育中的详细作用机制体系尚未完善,相关动物实验技术尚未成熟,国内外尚未出现相关临床实验。由于Hedgehog即参与骨发育,又参与某些胚胎组织的血管重新形成和成年哺乳动物的血管发生,因而有望在修复骨缺损的同时解决骨组织工程血管化的问题。Hedgehog信号通路的研究在骨组织工程及临床基因干预治疗等领域有广阔的前景。
BACKGROUND：Hedgehog, as an important regulatory factor in bone growth, has been recently focused for its mechanism in bone growth. OBJECTIVE：To introduce the mechanisms of Hedgehog in cartilage and skeleton development and the relationship between the hedgehog signalling pathway and bone disease and to investigate the research progress in Hedgehog signalling pathway in bone development. METHODS：A computer-based online search in PubMed and CNKI database was performed using key words of ＂Hedgehog, bone development, mesenchymal stem cells, cartilage, osteogenesis, bone defects＂ in English and Chinese respectively. The published dates are limited between January 1994 and December 2010. Researches related to this review purpose were included. Irrelevant or repetitive articles were excluded. Then 31 articles were selected according to the inclusive criteria for the review. RESULTS AND CONCLUSTION：Hedgehog is closely related to almost every stages of bone development, including differentiations from mesenchymal stem cells to osteocyte, formations of cartilage tissue and skeleton and other aspects. Aberrant Hedgehog signalling pathway will lead to severe developmental disorders including bone deformities and bone defects. However, the detailed mechanism regarding Hedgehog signalling pathway remains poorly understood and techniques regarding related animal experiments have not yet been so sophisticated that no clinical trials are performed both at home and abroad. Hedgehog is involved in osteoblast development and vascularisation in both embryogenesis and adult mammalian. For this reason, it is potential to solve the problem of angiogenesis while repairing bone defect in bone tissue engineering. With continuous researches on the Hedgehog signalling pathway in depth, Hedgehog signalling pathway has prosperous future in the areas of bone tissue engineering and clinical gene therapy.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第37期6991-6994,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30973069
30672146)资助
课题名称分别为:Ihh在组织工程骨构建中作用和机制研究
Wnt7b的信号传导在骨发育中的作用和机制研究~~
