摘要
目的:建立一种应用高效液相色谱-串联质谱(HPLC-MS/MS)同时测定植物源性食品中4种噻唑类杀菌剂的新检测方法。方法:采用乙腈提取试样中的噻唑类杀菌剂残留,提取液经浓缩,Sep-pack Vac复合固相萃取小柱净化,用HPLC-MS/MS进行定量分析。结果:4种噻唑类杀菌剂得到良好分离,方法的线性范围为20μg/L～100μg/L,检出限均为10μg/kg,回收率为64%～115%,相对标准偏差(RSD)为2.8%～12.5%。结论:实验结果表明,该方法简便、快速、灵敏、准确,各项技术指标满足检测植物源性食品中4种噻唑类杀菌剂的监测需求。
Objective：To establish a method for simultaneous determination of four thiazole fungicides in food of plant origin by HPLC-MS/MS.Methods： The thiazole fungicide residues in samples were extracted by acetonitrile.After concentration,the extract was cleaned up by passing through a Sep-pack Vac SPE cartridge,and then analysed by HPLC-MS/MS.Results： Four thiazole fungicides were well separated.The linear range of the calibration curve was 20 μg/L^100 μg/L,the detection limits of 4 thiazole fungicides were 10 μg/kg,the recovery range was 64%~115%,and the RSD range was 2.8%~12.5%.Conclusion： The method is simple,rapid,sensitive and accurate.The technical specifications are suitable for the detection of four thiazole fungicides in food of plant origin.
出处
《中国卫生检验杂志》
CAS
2011年第10期2418-2420,共3页
Chinese Journal of Health Laboratory Technology
关键词
液质联用
噻唑类杀菌剂
残留
固相萃取
HPLC-MS/MS
Thiazole fungicide
Residue
Solid phase extraction
