摘要
目的:建立水产品中泰乐菌素残留量的高效液相色谱测定方法。方法:样品在碱性条件下用乙酸乙酯提取,提取液旋转蒸干后,经正己烷脱脂,SCX柱固相萃取净化,乙腈与磷酸盐缓冲液为流动相梯度洗脱,外标法定量。结果:泰乐菌素在0.050μg/ml～5.00μg/ml内呈良好线性关系,相关系数r=0.9998。检出限为30μg/kg,定量限为100μg/kg。在50μg/kg～500μg/kg添加水平下回收率为72.4%～106%,相对标准偏差均小于10%。结论:该方法的灵敏度和重复性均满足水产品中泰乐菌素药物残留量检测需要。
Objective：To establish a HPLC method for determination of tylosin residue in fishery products.Methods： The samples were extracted by ethyl acetate at pH 8.0 medium,after condensed with rotary evaporation,the sample was extracted for defatting by hexane,and purified with SCX solid-phase extraction column.The target compound was separated with gradient mobile phase consisting of acetonitrile-phosphate buffer solution,and quantified by external standard method.Results： The method showed a good linearity within the range of 0.050 μg/ml^5.00 μg/ml with a correlation coefficient of 0.9998.The detection limit was 30 μg/kg and the quantification limits were 100 μg/kg.The mean recoveries at spiked concentration of 50 μg/kg^500 μg/kg were in the range of 72.4%~106%,and the relative standard deviations were less than 10%.Conclusion： The sensitivity and repeatability of the method could meet the analysis requirements for determination of tylosin residues in fishery products.
出处
《中国卫生检验杂志》
CAS
2011年第10期2371-2372,2376,共3页
Chinese Journal of Health Laboratory Technology
基金
农业部兽药残留研究项目(2006-52)
