摘要
目的:建立检测香港海鸥形菌的实时荧光PCR方法。方法:根据香港海鸥形菌以16SrDNA基因设计探针和引物,建立检测香港海鸥形菌荧光PCR方法,并对建立的方法进行特异性、灵敏性的评价。结果:检测体系灵敏度高,菌液的最低检出限可达40 CFU/反应体系;特异性好,对329株细菌的检测符合率达100%。结论:建立的实时PCR检测方法可应用于食源性致病菌中香港海鸥形菌的快速检测。
Objective：To develop a real-time PCR assay for the rapid detection of Laribacter hongkongensis.Methods： Based on the sequences of 16SrDNA gene published on GeneBank,primers and molecular beacon probe were designed,the real-time PCR assay for the rapid detection of Laribacter hongkongensis was developed.Then the specificity,sensitivity of the real-time PCR method were evaluated.Results： The detection system had high sensitivity and the lowest detection limit was 40CFU/reaction.There was no cross-reaction with other bacteria as control.The assay was tested against 329 strains,no false signals were observed and the result was consistence with the traditional method.Conclusion： The real-time PCR assay could be applied to the rapid detection of Laribacter hongkongensis on the foodborne pathogens.
出处
《中国卫生检验杂志》
CAS
2011年第10期2368-2370,共3页
Chinese Journal of Health Laboratory Technology
基金
深圳市科技局(201003125)
