摘要
为对犬干扰素进行研究,试验采用PCR法将犬IFN-α1基因从基因组DNA克隆到pGEM-T载体,并进行测序分析。结果表明:犬IFN-α1基因ORF大小为564 bp,编码187个氨基酸,前23个为信号肽;成熟蛋白理论分子质量为19 ku,等电点为6.217,有4个潜在磷酸化位点1、个N糖基化位点和4个半胱氨酸残基,大部分为亲水区;经二级、三级结构预测,该蛋白主要由5段α螺旋组成;犬IFN-α1基因与貉子、赤狐的同源性最高,为98%,在进化树中处于同一分支;在干扰素多肽链中,15L、18L、77W、96L9、9C1、24F、131L、137S、140AWE1421、45R和150R在所有分析物种中高度保守。
To conduct the research to the canine interferon-α （IFN- α） , IFN -α1 gone of canine was cloned to pGEM -T vector from gcnomic DNA using the PCR method and was sequenced. The results showed that it was consisted of 564 bp of ORF and encoded for a 187 amino acids,the first 23 amino acids were signal peptides, the mature protein had a molecular mass of 19 ku and the isoelectric point of 6. 217. It had 4 potential casein kinase Ⅱ phosphorylation sites, 1 N -glycosylation site and 4 cysteine residues, and most of which were the hydrophilic regions. The protein was composed mainly of five sections of α - helix according to the secondary and tertiary structure prediction. The IFN -α1 gene of canine had the maximum similarity of 98% with that of raccoon dog and red fox, grouped into the same branch in the phylogenetic tree base on IFN -α1 gene. In the polypeptide chain of IFN -α, the amino acid residues at positions 15L, 18L, 77W, 96L, 99C, 124F, 131 L, 137S, 140AWE142, 145R and 150R were highly conserved in all analyzed species.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2011年第10期20-23,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
沈阳农业大学青年教师科研基金项目(2006210)
