摘要
通过单因素实验及响应面方法对黑曲霉菌株产木聚糖酶发酵条件进行了研究,结果表明:当培养基为麸皮40g/L,(NH4)2 SO4 4g/L,KH2PO4 3g/L,MgSO4 0.8g/L,吐温80 1g/L;初始pH值5.0,接种量8.0%,培养温度30℃;此条件下发酵木聚糖酶酶活达1456.27IU/mL。
The fermentation conditions of aspergillus niger producing xylanase were studied by single facter experiment and response surface method. The results were as follows ： bran 40 g / L , （ NH4 ） 2 SO4 4g/L, KH2PO4 4g/L, MgSO4 0.8g/L, Tween 80 1 g/L, initial pH value 5.0, inoculation volume 8.0%, temperature 30℃, under these conditions, the activity of xylanase was reached 1456.27IU/mL.
出处
《山东化工》
CAS
2011年第9期8-12,19,共6页
Shandong Chemical Industry
基金
国家科技支撑计划项目(2007BAD66B01)
关键词
黑曲霉
木聚糖酶
酶活力
培养基
发酵条件
响应面
aspergillus niger
xylanase
enzyme activity
culture medium
fermentation condition
response surface
